Highly efficient generation of T-DNA insertion lines and isolation of flanking sequence tags (FSTs) of Brachypodium distachyon
Sign Up to like & getrecommendations! Published in 2018 at "Plant Biotechnology Reports"
DOI: 10.1007/s11816-018-0489-4
Abstract: Brachypodium distachyon (Brachypodium) has been developed as a model system for the temperate grasses. Hence, establishing a large insertion mutant population and identifying T-DNA insertion sites are imperative for functional genomics studies. Currently, Brachypodium has… read more here.
Keywords: dna insertion; flanking sequence; brachypodium; dna ... See more keywords
Polymerization retardation isothermal amplification strategy enables the sensitive and facile investigation of the flanking sequence preference of ten-eleven translocation 2 protein.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.02.038
Abstract: Active DNA demethylation process critically relies on the intrinsic properties of ten-eleven translocation proteins (Tets), particularly the flanking sequence preference. Challenged by the fact that the proximate bases to the 5-methylcytosine (5mC) are multitudinous and… read more here.
Keywords: sequence preference; preference; strategy; flanking sequence ... See more keywords
The impact of flanking sequence features on DNA CpG methylation
Sign Up to like & getrecommendations! Published in 2021 at "Computational biology and chemistry"
DOI: 10.1016/j.compbiolchem.2021.107480
Abstract: Epigenetics and DNA methylation play a pivotal role in many processes of the cell and we often observe that an aberrant methylation pattern characterizes pathologies. In this work we investigate the role that the flanking… read more here.
Keywords: methylation; features dna; impact flanking; flanking sequence ... See more keywords
Deep enzymology studies on DNA methyltransferases reveal novel connections between flanking sequences and enzyme activity.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2021.167186
Abstract: DNA interacting enzymes recognize their target sequences embedded in variable flanking sequence context. The influence of flanking sequences on enzymatic activities of DNA methyltransferases (DNMTs) can be systematically studied with "deep enzymology" approaches using pools… read more here.
Keywords: flanking sequences; dna; deep enzymology; enzymology ... See more keywords
Preferential self-interaction of DNA methyltransferase DNMT3A subunits containing the R882H cancer mutation leads to dominant changes of flanking sequence preferences.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2022.167482
Abstract: Somatic R882H DNMT3A mutations occur frequently in AML, but their pathogenic mechanism is unclear. As R882H mutations usually are heterozygous, wildtype (WT) and R882H subunits co-exist in affected cells. R882 is located in the RD… read more here.
Keywords: containing r882h; flanking sequence; dnmt3a; sequence preferences ... See more keywords