Flavin‐N5 Covalent Intermediate in a Nonredox Dehalogenation Reaction Catalyzed by an Atypical Flavoenzyme
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201700594
Abstract: The flavin‐dependent enzyme 2‐haloacrylate hydratase (2‐HAH) catalyzes the conversion of 2‐chloroacrylate, a major component in the manufacture of acrylic polymers, to pyruvate. The enzyme was expressed in Escherichia coli, purified, and characterized. 2‐HAH was shown… read more here.
Keywords: flavoenzyme; flavin covalent; covalent; flavin ... See more keywords
Broadening the Scope of the Flavin‐Tag Method by Improving Flavin Incorporation and Incorporating Flavin Analogs
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200144
Abstract: Methods for facile site‐selective modifications of proteins are in high demand. We have recently shown that a flavin transferase can be used for site‐specific covalent attachment of a chromo‐ and fluorogenic flavin (FMN) to any… read more here.
Keywords: flavin tag; flavin incorporation; tag method; flavin ... See more keywords
Asymmetric catalysis by flavin-dependent halogenases.
Sign Up to like & getrecommendations! Published in 2023 at "Chirality"
DOI: 10.1002/chir.23550
Abstract: In nature, flavin-dependent halogenases (FDHs) catalyze site-selective chlorination and bromination of aromatic natural products. This ability has led to extensive efforts to engineer FDHs for selective chlorination, bromination, and iodination of electron rich aromatic compounds.… read more here.
Keywords: flavin dependent; catalysis; asymmetric catalysis; dependent halogenases ... See more keywords
Mechanism of Nitrone Formation by a Flavin-Dependent Monooxygenase
Sign Up to like & getrecommendations! Published in 2024 at "Biochemistry"
DOI: 10.1021/acs.biochem.3c00656
Abstract: OxaD is a flavin-dependent monooxygenase (FMO) responsible for catalyzing the oxidation of an indole nitrogen atom, resulting in the formation of a nitrone. Nitrones serve as versatile intermediates in complex syntheses, including challenging reactions like… read more here.
Keywords: flavin; flavin dependent; dependent monooxygenase; mechanism ... See more keywords
Oxidation of Flavin by Molecular Oxygen: Computational Insights into a Possible Radical Mechanism
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DOI: 10.1021/acsomega.4c00307
Abstract: As a highly electrophilic moiety capable of oxidizing a variety of small organic molecules and biomolecules, flavin is an important prosthetic group in many enzymes. Upon oxidation of the substrate, flavin is converted into its… read more here.
Keywords: flavin; oxidation; molecular oxygen; radical mechanism ... See more keywords
Advanced flavin catalysts elaborated with polymers
Sign Up to like & getrecommendations! Published in 2018 at "Polymer Journal"
DOI: 10.1038/s41428-018-0089-8
Abstract: AbstractA variety of biological redox reactions are mediated by flavoenzymes due to the unique redox activity of isoalloxazine ring systems, which are found in flavin cofactors. In the field of synthetic organic chemistry, the term… read more here.
Keywords: flavin catalysts; polymer; flavin; catalytic activity ... See more keywords
pH modulates efficiency of singlet oxygen production by flavin cofactors
Sign Up to like & getrecommendations! Published in 2024 at "RSC Advances"
DOI: 10.1039/d4ra05540c
Abstract: Flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) are frequently used interchangeably in the catalysis of various reactions as part of flavoenzymes because they have the same functional component, the isoalloxazine ring. However, they differ… read more here.
Keywords: flavin; oxygen; singlet oxygen; fmn ... See more keywords
A single hydrogen bond that tunes flavin redox reactivity and activates it for modification
Sign Up to like & getrecommendations! Published in 2024 at "Chemical Science"
DOI: 10.1039/d4sc01642d
Abstract: Electron bifurcation produces high-energy products based on less energetic reagents. This feat enables biological systems to exploit abundant mediocre fuel to drive vital but demanding reactions, including nitrogen fixation and CO2 capture. Thus, there is… read more here.
Keywords: flavin; chemistry; hydrogen bond; bond ... See more keywords
Evolutionary and molecular foundations of multiple contemporary functions of the nitroreductase superfamily
Sign Up to like & getrecommendations! Published in 2017 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1706849114
Abstract: Significance Functionally diverse enzyme superfamilies are sets of homologs that conserve a structural fold and mechanistic details but perform various distinct chemical reactions. What are the evolutionary routes by which ancestral proteins diverge to produce… read more here.
Keywords: molecular foundations; flavin; enzyme superfamilies; foundations multiple ... See more keywords
An uncharacteristically low-potential flavin governs the energy landscape of electron bifurcation
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2117882119
Abstract: Significance Nature has long been an inspiration for materials design, as it exemplifies exquisite control of both matter and energy. Electron bifurcation, a mechanism employed in biological systems to drive thermodynamically unfavorable and energetically challenging… read more here.
Keywords: energy landscape; energy; electron bifurcation; electron ... See more keywords
Ultrafast photooxidation of protein-bound anionic flavin radicals
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118924119
Abstract: Significance Flavoproteins are colored proteins involved in a large variety of biochemical reactions. They can perform photochemical reactions, which are increasingly exploited for bioengineering new protein-derived photocatalysts. In particular, light-induced reduction of the resting oxidized… read more here.
Keywords: protein bound; anionic flavin; flavin radicals; bound anionic ... See more keywords