Flavin‐N5 Covalent Intermediate in a Nonredox Dehalogenation Reaction Catalyzed by an Atypical Flavoenzyme
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201700594
Abstract: The flavin‐dependent enzyme 2‐haloacrylate hydratase (2‐HAH) catalyzes the conversion of 2‐chloroacrylate, a major component in the manufacture of acrylic polymers, to pyruvate. The enzyme was expressed in Escherichia coli, purified, and characterized. 2‐HAH was shown… read more here.
Keywords: flavoenzyme; flavin covalent; covalent; flavin ... See more keywords
Broadening the Scope of the Flavin‐Tag Method by Improving Flavin Incorporation and Incorporating Flavin Analogs
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200144
Abstract: Methods for facile site‐selective modifications of proteins are in high demand. We have recently shown that a flavin transferase can be used for site‐specific covalent attachment of a chromo‐ and fluorogenic flavin (FMN) to any… read more here.
Keywords: flavin tag; flavin incorporation; tag method; flavin ... See more keywords
Asymmetric catalysis by flavin-dependent halogenases.
Sign Up to like & getrecommendations! Published in 2023 at "Chirality"
DOI: 10.1002/chir.23550
Abstract: In nature, flavin-dependent halogenases (FDHs) catalyze site-selective chlorination and bromination of aromatic natural products. This ability has led to extensive efforts to engineer FDHs for selective chlorination, bromination, and iodination of electron rich aromatic compounds.… read more here.
Keywords: flavin dependent; catalysis; asymmetric catalysis; dependent halogenases ... See more keywords
Advanced flavin catalysts elaborated with polymers
Sign Up to like & getrecommendations! Published in 2018 at "Polymer Journal"
DOI: 10.1038/s41428-018-0089-8
Abstract: AbstractA variety of biological redox reactions are mediated by flavoenzymes due to the unique redox activity of isoalloxazine ring systems, which are found in flavin cofactors. In the field of synthetic organic chemistry, the term… read more here.
Keywords: flavin catalysts; polymer; flavin; catalytic activity ... See more keywords
Evolutionary and molecular foundations of multiple contemporary functions of the nitroreductase superfamily
Sign Up to like & getrecommendations! Published in 2017 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1706849114
Abstract: Significance Functionally diverse enzyme superfamilies are sets of homologs that conserve a structural fold and mechanistic details but perform various distinct chemical reactions. What are the evolutionary routes by which ancestral proteins diverge to produce… read more here.
Keywords: molecular foundations; flavin; enzyme superfamilies; foundations multiple ... See more keywords
An uncharacteristically low-potential flavin governs the energy landscape of electron bifurcation
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2117882119
Abstract: Significance Nature has long been an inspiration for materials design, as it exemplifies exquisite control of both matter and energy. Electron bifurcation, a mechanism employed in biological systems to drive thermodynamically unfavorable and energetically challenging… read more here.
Keywords: energy landscape; energy; electron bifurcation; electron ... See more keywords
Ultrafast photooxidation of protein-bound anionic flavin radicals
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118924119
Abstract: Significance Flavoproteins are colored proteins involved in a large variety of biochemical reactions. They can perform photochemical reactions, which are increasingly exploited for bioengineering new protein-derived photocatalysts. In particular, light-induced reduction of the resting oxidized… read more here.
Keywords: protein bound; anionic flavin; flavin radicals; bound anionic ... See more keywords
On the Cost and Role of Secreted Flavin Cofactors for Anaerobic Respiration of Fumarate and Urocanate by Shewanella oneidensis.
Sign Up to like & getrecommendations! Published in 2019 at "Applied and environmental microbiology"
DOI: 10.1128/aem.00852-19
Abstract: Shewanella oneidensis strain MR-1, a facultative anaerobe and model organism for dissimilatory metal reduction, uses a periplasmic flavocytochrome, FccA, as both a terminal fumarate reductase and as a periplasmic electron transfer hub for extracellular respiration… read more here.
Keywords: respiration; shewanella oneidensis; flavin; fumarate ... See more keywords
The flavin monooxygenase Bs3 triggers cell death in plants, impairs growth in yeast and produces H2O2 in vitro
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0256217
Abstract: The pepper resistance gene Bs3 triggers a hypersensitive response (HR) upon transcriptional activation by the corresponding effector protein AvrBs3 from the bacterial pathogen Xanthomonas. Expression of Bs3 in yeast inhibited proliferation, demonstrating that Bs3 function… read more here.
Keywords: bs3; bs3 triggers; flavin; h2o2 ... See more keywords
Unique Features and Anti-microbial Targeting of Folate- and Flavin-Dependent Methyltransferases Required for Accurate Maintenance of Genetic Information
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2018.00918
Abstract: Comparative genome analyses have led to the discovery and characterization of novel flavin- and folate-dependent methyltransferases that mainly function in DNA precursor synthesis and post-transcriptional RNA modification by forming (ribo) thymidylate and its derivatives. Here… read more here.
Keywords: methylation; dependent methyltransferases; flavin dependent; anti microbial ... See more keywords
Enhanced Solid-State Fluorescence of Flavin Derivatives by Incorporation in the Metal-Organic Frameworks MIL-53(Al) and MOF-5
Sign Up to like & getrecommendations! Published in 2023 at "Molecules"
DOI: 10.3390/molecules28062877
Abstract: The flavin derivatives 10-methyl-isoalloxazine (MIA) and 6-fluoro-10-methyl-isoalloxazine (6F-MIA) were incorporated in two alternative metal-organic frameworks, (MOFs) MIL-53(Al) and MOF-5. We used a post-synthetic, diffusion-based incorporation into microcrystalline MIL-53 powders with one-dimensional (1D) pores and an… read more here.
Keywords: flavin mof; state; mof; mia ... See more keywords