Predicting the Substrate Scope of the Flavin‐Dependent Halogenase BrvH
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.202000444
Abstract: The recently described flavin‐dependent halogenase BrvH is able to catalyse both the bromination and chlorination of indole, but shows significantly higher bromination activity. BrvH was annotated as a tryptophan halogenase, but does not accept tryptophan… read more here.
Keywords: flavin dependent; halogenase; dependent halogenase; model ... See more keywords
Engineered Bacterial Flavin‐Dependent Monooxygenases for the Regiospecific Hydroxylation of Polycyclic Phenols
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202100480
Abstract: 4‐Hydroxyphenylacetate 3‐hydroxylase (4HPA3H), a flavin‐dependent monooxygenase from E. coli that catalyzes the hydroxylation of monophenols to catechols, was modified by rational redesign to convert also more bulky substrates, especially phenolic natural products like phenylpropanoids, flavones… read more here.
Keywords: flavin dependent; monooxygenases regiospecific; bacterial flavin; dependent monooxygenases ... See more keywords
Asymmetric catalysis by flavin-dependent halogenases.
Sign Up to like & getrecommendations! Published in 2023 at "Chirality"
DOI: 10.1002/chir.23550
Abstract: In nature, flavin-dependent halogenases (FDHs) catalyze site-selective chlorination and bromination of aromatic natural products. This ability has led to extensive efforts to engineer FDHs for selective chlorination, bromination, and iodination of electron rich aromatic compounds.… read more here.
Keywords: flavin dependent; catalysis; asymmetric catalysis; dependent halogenases ... See more keywords
Comparative Activity-Based Flavin-Dependent Oxidase Profiling.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6439-0_7
Abstract: Activity-based protein profiling (ABPP) has become a powerful chemoproteomic technology allowing for the dissection of complex ligand-protein interactions in their native cellular environment. One of the biggest challenges for ABPP is the extension of the… read more here.
Keywords: flavin dependent; comparative activity; activity based; dependent oxidase ... See more keywords
Flavin-dependent epitranscriptomic world.
Sign Up to like & getrecommendations! Published in 2017 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2017.06.011
Abstract: RNAs molecules fulfill key roles in the expression and regulation of the genetic information stored within the DNA chromosomes. In addition to the four canonical bases, U, C, A and G, RNAs harbor various chemically… read more here.
Keywords: chemistry; dependent epitranscriptomic; flavin dependent; epitranscriptomic world ... See more keywords
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00493
Abstract: Acinetobacter baumannii is a Gram-negative opportunistic pathogen that causes nosocomial infections, especially among immunocompromised individuals. The rise of multidrug resistant strains of A. baumannii has limited the use of standard antibiotics, highlighting a need for… read more here.
Keywords: flavin dependent; fbsi; acinetobacter baumannii;
Structural and Biochemical Characterization of the Flavin-Dependent Siderophore-Interacting Protein from Acinetobacter baumannii
Sign Up to like & getrecommendations! Published in 2021 at "ACS Omega"
DOI: 10.1021/acsomega.1c03047
Abstract: Acinetobacter baumannii is an opportunistic pathogen with a high mortality rate due to multi-drug-resistant strains. The synthesis and uptake of the iron-chelating siderophores acinetobactin (Acb) and preacinetobactin (pre-Acb) have been shown to be essential for… read more here.
Keywords: flavin dependent; dependent siderophore; pre; acinetobacter baumannii ... See more keywords
Flavin-Dependent Monooxygenase-Mediated 1,2-Oxazine Construction via Meisenheimer Rearrangement in the Biosynthesis of Paeciloxazine.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c00881
Abstract: The [1,2]-Meisenheimer rearrangement is well known as the [1,2]-migration of an O-substituted hydroxylamine from a tertiary amine N-oxide, and it is frequently employed in organic synthesis to enforce adjacent carbon oxidation or install a 1,2-oxazine… read more here.
Keywords: meisenheimer rearrangement; rearrangement; dependent monooxygenase; flavin dependent ... See more keywords
Directed Evolution of Flavin-Dependent Halogenases for Site- and Atroposelective Halogenation of 3-Aryl-4(3H)-Quinazolinones via Kinetic or Dynamic Kinetic Resolution.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c07422
Abstract: In this study, we engineer a variant of the flavin-dependent halogenase RebH that catalyzes site- and atroposelective halogenation of 3-aryl-4(3H)-quinazolinones via kinetic or dynamic kinetic resolution. The required directed evolution uses a combination of random… read more here.
Keywords: flavin dependent; atroposelective halogenation; halogenation aryl; site atroposelective ... See more keywords
Flavin-dependent halogenases catalyze enantioselective olefin halocyclization
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-23503-3
Abstract: Halocyclization of alkenes is a powerful bond-forming tool in synthetic organic chemistry and a key step in natural product biosynthesis, but catalyzing halocyclization with high enantioselectivity remains a challenging task. Identifying suitable enzymes that catalyze… read more here.
Keywords: dependent halogenases; flavin dependent; enantioselective olefin; catalyze enantioselective ... See more keywords
Deciphering the evolution of flavin-dependent monooxygenase stereoselectivity using ancestral sequence reconstruction.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2218248120
Abstract: Controlling the selectivity of a reaction is critical for target-oriented synthesis. Accessing complementary selectivity profiles enables divergent synthetic strategies, but is challenging to achieve in biocatalytic reactions given enzymes' innate preferences of a single selectivity.… read more here.
Keywords: flavin dependent; ancestral sequence; reaction; stereoselectivity ... See more keywords