Molecular cloning and biochemical characterization of a new flavonoid glycosyltransferase from the aquatic plant lotus.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.01.099
Abstract: Lotus (Nelumbo nucifera Gaertn.), one of the earliest plants in angiosperms, is a perennial aquatic herb widely distributed throughout Eastern Asia. Quercetin and its glycosides are the most abundant phenolic compounds in lotus with multiple… read more here.
Keywords: aquatic plants; lotus; glycosyltransferase aquatic; glycosyltransferase ... See more keywords
Highly Promiscuous Flavonoid 3- O-Glycosyltransferase from Scutellaria baicalensis.
Sign Up to like & getrecommendations! Published in 2019 at "Organic letters"
DOI: 10.1021/acs.orglett.9b00524
Abstract: A highly regio-specific and donor-promiscuous 3- O-glycosyltransferase, Sb3GT1 (UGT78B4), was discovered from Scutellaria baicalensis. Sb3GT1 could accept five sugar donors (UDP-Glc/-Gal/-GlcNAc/-Xyl/-Ara) to catalyze 3- O-glycosylation of 17 flavonols, and the conversion rates could be >98%.… read more here.
Keywords: promiscuous flavonoid; scutellaria baicalensis; highly promiscuous; flavonoid glycosyltransferase ... See more keywords
Engineering the Entrance of a Flavonoid Glycosyltransferase Promotes the Glycosylation of Etoposide Aglycone.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00032
Abstract: Enzyme entrances, which function as the first molecular filters, influence substrate selectivity and enzymatic activity. Because of low binding affinities, engineering enzyme entrances that recognize non-natural substrates is a major challenge for artificial biocatalyst design.… read more here.
Keywords: entrance flavonoid; flavonoid glycosyltransferase; etoposide aglycone; engineering ... See more keywords