Correlation of intracellular oxygen and cell metabolism by simultaneous PLIM of phosphorescent TLD1433 and FLIM of NAD(P)H.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biophotonics"
DOI: 10.1002/jbio.201800085
Abstract: During photodynamic therapy (PDT), disruption of cell respiration and metabolic changes could be one of the first events. Photophysical characteristics of the photosensitizer (PS) and its specific redox potential define consumption of molecular oxygen followed… read more here.
Keywords: flim; oxygen; metabolism; pdt ... See more keywords
Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes.
Sign Up to like & getrecommendations! Published in 2018 at "Fertility and sterility"
DOI: 10.1016/j.fertnstert.2018.07.022
Abstract: OBJECTIVE To determine whether metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) identifies metabolic differences between normal oocytes and those with metabolic dysfunction. DESIGN Experimental study. SETTING Academic research laboratories. PATIENT(S) None.… read more here.
Keywords: flim; fluorescence; microscopy; dysfunction ... See more keywords
Quantitative real-time imaging of intracellular FRET biosensor dynamics using rapid multi-beam confocal FLIM
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-61478-1
Abstract: Fluorescence lifetime imaging (FLIM) is a quantitative, intensity-independent microscopical method for measurement of diverse biochemical and physical properties in cell biology. It is a highly effective method for measurements of Förster resonance energy transfer (FRET),… read more here.
Keywords: flim; quantitative real; biosensor; flim quantitative ... See more keywords
Optimization of FLIM imaging, fitting and analysis for auto-fluorescent NAD(P)H and FAD in cells and tissues.
Sign Up to like & getrecommendations! Published in 2020 at "Methods and applications in fluorescence"
DOI: 10.1088/2050-6120/ab6f25
Abstract: Increasingly, the auto-fluorescent coenzymes NAD(P)H and FAD are being tracked by multi-photon fluorescence lifetime microscopy (FLIM) and used as versatile markers for changes in mammalian metabolism. The cellular redox state of different cell model systems,… read more here.
Keywords: flim; analysis; nad fad; auto fluorescent ... See more keywords
Fluorescence lifetime imaging microscopy (FLIM) detects differences in metabolic signatures between euploid and aneuploid human blastocysts.
Sign Up to like & getrecommendations! Published in 2022 at "Human reproduction"
DOI: 10.1093/humrep/deac016
Abstract: STUDY QUESTION Can non-invasive imaging with fluorescence lifetime imaging microscopy (FLIM) detect metabolic differences in euploid versus aneuploid human blastocysts? SUMMARY ANSWER FLIM has identified significant metabolic differences between euploid and aneuploid blastocysts. WHAT IS… read more here.
Keywords: flim; fluorescence lifetime; microscopy; human blastocysts ... See more keywords
Abstract 15508: Random Forest Classifier-incoporated Intravascular Optical Coherence Tomography-fluorescence Lifetime Imaging (oct-flim) Provides Automated Characterization of Key Biochemical Components of Coronary Atherosclerotic Plaques
Sign Up to like & getrecommendations! Published in 2020 at "Circulation"
DOI: 10.1161/circ.142.suppl_3.15508
Abstract: Introduction: Fluorescence lifetime imaging (FLIm) allows label-free biochemical characterization of atheroma, however, multispectral FLIm produces massive data throughput making image interpretati... read more here.
Keywords: flim; characterization; fluorescence lifetime; lifetime imaging ... See more keywords