Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer
Sign Up to like & getrecommendations! Published in 2017 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23141
Abstract: Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell… read more here.
Keywords: red blood; rbc; blood cells; flow cytometer ... See more keywords
Comments on “Determination of Background, Signal‐to‐Noise, and Dynamic Range of a Flow Cytometer: A Novel Practical Method for Instrument Characterization and Standardization”
Sign Up to like & getrecommendations! Published in 2019 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23943
Abstract: The characterization of flow cytometer instrument performance can be used to help design and execute better assays but involves technical complexities that have their roots in the instrument hardware and software. A recent publication that… read more here.
Keywords: noise dynamic; background signal; signal noise; flow ... See more keywords
Response to: Comments on the publication “Determination of Background, Signal‐to‐Noise, and Dynamic Range of a Flow Cytometer: A Novel Practical Method for Instrument Characterization and Standardization” by Giesecke et al.
Sign Up to like & getrecommendations! Published in 2020 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23977
Abstract: IN the September 2017 issue of Cytometry A, we have described the use of an LED pulser to characterize a flow cytometer’s photodetector ́s response curve over a defined range of amplification, henceforth referred to… read more here.
Keywords: dynamic range; response; method; flow cytometer ... See more keywords
Hydrodynamic flow cytometer performance enhancement by two-dimensional acoustic focusing
Sign Up to like & getrecommendations! Published in 2020 at "Biomedical Microdevices"
DOI: 10.1007/s10544-020-00481-9
Abstract: Conventional flow cytometers employ hydrodynamic focusing method to insure detection accuracy by forcing cells go through detected position. However, an increased flow velocity will significantly reduce detection precision due to a fact that cells will… read more here.
Keywords: acoustic focusing; performance; focusing; flow cytometer ... See more keywords
Comparison of Free Zinc Levels Determined by Fluorescent Probes in THP1 Cells Using Microplate Reader and Flow Cytometer
Sign Up to like & getrecommendations! Published in 2020 at "Biological Trace Element Research"
DOI: 10.1007/s12011-020-02355-w
Abstract: Free zinc is involved in signal transduction within mammalian cells, acting as a second messenger. Gold standard for its analysis is currently the use of metal-responsive fluorescent probes. The present study elucidates the impact of… read more here.
Keywords: free zinc; zinc; flow cytometer; fluorescent probes ... See more keywords
A high-throughput technique to map cell images to cell positions using a 3D imaging flow cytometer
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118068119
Abstract: Significance This article demonstrates a high-throughput technique to map cell images to cell positions. The technology uses a three-dimensional (3D) imaging flow cytometer to record multiparameter 3D cell images at a throughput of 1,000 cells/s… read more here.
Keywords: cell; cell images; imaging flow; flow cytometer ... See more keywords
Evaluation of the sysmex UF-5000 fluorescence flow cytometer as a screening platform for ruling out urinary tract infections in elderly patients presenting at the Emergency Department
Sign Up to like & getrecommendations! Published in 2021 at "Scandinavian Journal of Clinical and Laboratory Investigation"
DOI: 10.1080/00365513.2021.1929441
Abstract: Abstract In this study, we evaluated the performance of the flow cytometer-based Sysmex UF-5000 automated urine analyzer as a screening tool for ruling out urinary tract infections in elderly patients presenting at the emergency department.… read more here.
Keywords: urinary tract; tract infections; elderly patients; sysmex 5000 ... See more keywords
Multi-color flow cytometer with PIC-based structured illumination.
Sign Up to like & getrecommendations! Published in 2022 at "Biomedical optics express"
DOI: 10.1364/boe.464903
Abstract: We demonstrate a flow cytometer in which structured light illumination is used to attribute fluorescent and scattering signals to their excitation wavelength. A suitable multi-color light source emitting structured illumination patterns at 405, 488, 561… read more here.
Keywords: structured illumination; flow cytometer; multi color; cytometer ... See more keywords
High-Precision Lens-Less Flow Cytometer on a Chip
Sign Up to like & getrecommendations! Published in 2018 at "Micromachines"
DOI: 10.3390/mi9050227
Abstract: We present a flow cytometer on a microfluidic chip that integrates an inline lens-free holographic microscope. High-speed cell analysis necessitates that cells flow through the microfluidic channel at a high velocity, but the image sensor… read more here.
Keywords: high precision; flow cytometer; method; cytometer ... See more keywords