T-Cell Receptor Gene Rearrangement Clonality, Flow Cytometry Status, and Associated Outcomes in Early-Stage Cutaneous T-Cell Lymphoma.
Sign Up to like & getrecommendations! Published in 2021 at "JAMA dermatology"
DOI: 10.1001/jamadermatol.2021.2191
Abstract: Importance The prognostic significance of clonal T-cell receptor (TCR) rearrangement or low-level blood involvement as assessed by flow cytometry for patients with early-stage cutaneous T-cell lymphoma (CTCL) is not clear. Objective To assess the association… read more here.
Keywords: cytometry; early stage; clonality; flow cytometry ... See more keywords
Building predictive disease models using extracellular vesicle microscale flow cytometry and machine learning
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13362
Abstract: Extracellular vesicles (EVs) are highly abundant in human biofluids, containing a repertoire of macromolecules and biomarkers representative of the tissue of origin. EVs released by tumours can communicate key signals both locally and to distant… read more here.
Keywords: disease; microscale flow; machine learning; flow cytometry ... See more keywords
Integration of multiparameter flow cytometry score improves prognostic stratification provided by standard models in primary myelofibrosis
Sign Up to like & getrecommendations! Published in 2022 at "American Journal of Hematology"
DOI: 10.1002/ajh.26548
Abstract: Prognostic modeling in myelofibrosis (MF) has classically pursued the integration of informative clinical and hematological parameters to separate patients' categories with different outcomes. Modern stratification includes also genetic data from karyotype and mutations. However, some… read more here.
Keywords: prognostic stratification; flow cytometry; multiparameter flow; stratification ... See more keywords
Analysis of Cellular DNA Content by Flow Cytometry
Sign Up to like & getrecommendations! Published in 2017 at "Current Protocols in Immunology"
DOI: 10.1002/cpim.36
Abstract: Cellular DNA content can be measured by flow cytometry with the aim of : (1) revealing cell distribution within the major phases of the cell cycle, (2) estimating frequency of apoptotic cells with fractional DNA… read more here.
Keywords: flow cytometry; cellular dna; dna content; dna ... See more keywords
Flow cytometry based rapid duplexed immunoassay for fusarium mycotoxins
Sign Up to like & getrecommendations! Published in 2017 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23018
Abstract: At small food processing facilities, the most frequently used test to determine if grain‐derived mycotoxin concentrations are compliant with legal limits is the enzyme‐linked immunosorbent assay (ELISA). Each kit is designed to detect one of… read more here.
Keywords: based rapid; duplexed immunoassay; flow cytometry; rapid duplexed ... See more keywords
Mass cytometry: The time to settle down
Sign Up to like & getrecommendations! Published in 2017 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23032
Abstract: MASS cytometry (CyTOF) technology was first described by Bandura et al. in 2009 (1) boosting the number of measurable markers per single cell and revolutionizing the flow cytometry field toward a horizon of a theoretical… read more here.
Keywords: mass cytometry; cytometry; issue page; flow cytometry ... See more keywords
Development of a bead‐based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry
Sign Up to like & getrecommendations! Published in 2017 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23170
Abstract: Quantifying cytokines is extremely important in studies of host–pathogen interactions. Multiplex assays are commercially available but only for human and mouse cytokines. Here a method for the simultaneous quantification of five important bovine cytokines IFNγ,… read more here.
Keywords: culture supernatants; bovine cytokines; simultaneous quantification; flow cytometry ... See more keywords
Shapiro's seventh law
Sign Up to like & getrecommendations! Published in 2018 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23579
Abstract: WHEN late at night (as I imagine it), Howard Shapiro finally came out of his laboratory, where he spent the whole day, he was carrying a massive ring binder in his hands to present the… read more here.
Keywords: cell; analysis; seventh law; flow cytometry ... See more keywords
Simultaneous Analysis of Phenotype and Cytogenetics Using Imaging Flow Cytometry: Time to Teach Old Dogs New Tricks
Sign Up to like & getrecommendations! Published in 2019 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23776
Abstract: THE importance of cytogenetic abnormalities in clinical diagnosis and response has been well established in particular for hematological malignancies with the diagnostic tools being microscopy-based karyotyping or fluorescence in situ hybridization (FISH). For chronic lymphocytic… read more here.
Keywords: spot; imaging flow; hybridization; intensity ... See more keywords
An R‐Derived FlowSOM Process to Analyze Unsupervised Clustering of Normal and Malignant Human Bone Marrow Classical Flow Cytometry Data
Sign Up to like & getrecommendations! Published in 2019 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23897
Abstract: Multiparameter flow cytometry (MFC) is a powerful and versatile tool to accurately analyze cell subsets, notably to explore normal and pathological hematopoiesis. Yet, mostly supervised subjective strategies are used to identify cell subsets in this… read more here.
Keywords: bone marrow; cytometry; human bone; flow cytometry ... See more keywords
OMIP‐064: A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells
Sign Up to like & getrecommendations! Published in 2020 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24031
Abstract: This 27‐color flow cytometry panel was developed in order to assess immunological changes over the course of an immunization and challenge regimen in two experimental malaria vaccine trials. The aim of the study was to… read more here.
Keywords: cytometry panel; panel detect; flow cytometry; panel ... See more keywords