Reduced surface adsorption in 3D printed acrylonitrile butadiene styrene micro free‐flow electrophoresis devices
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201900179
Abstract: We have 3D printed and fabricated micro free‐flow electrophoresis (µFFE) devices in acrylonitrile butadiene styrene (ABS) that exhibit minimal surface adsorption without requiring additional surface coatings or specialized buffer additives. 2D, nano LC–micro free flow… read more here.
Keywords: surface adsorption; adsorption; flow electrophoresis; free flow ... See more keywords
Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology
Sign Up to like & getrecommendations! Published in 2020 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202000149
Abstract: The increasing resolution of three‐dimensional (3D) printing offers simplified access to, and development of, microfluidic devices with complex 3D structures. Therefore, this technology is increasingly used for rapid prototyping in laboratories and industry. Microfluidic free… read more here.
Keywords: production; electrophoresis; flow electrophoresis; free flow ... See more keywords
Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7389-7_8
Abstract: Free-flow electrophoresis (FFE) is a technique for separation of proteins, peptides, organelles, and cells. With zone electrophoresis (ZE-FFE), organelles are separated according to surface charge. The ER is the only remaining major cellular compartment in… read more here.
Keywords: surface charge; electrophoresis; flow electrophoresis; free flow ... See more keywords
Current advances and challenges in microfluidic free-flow electrophoresis-A critical review.
Sign Up to like & getrecommendations! Published in 2017 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2017.08.017
Abstract: The research field on microfluidic free-flow electrophoresis has developed vast amounts of devices, methods, applications and raised new questions, often in analogy to conventional techniques from which it derives. Most efforts have been employed on… read more here.
Keywords: flow electrophoresis; free flow; review; microfluidic free ... See more keywords
Characterization of the acidic species of a monoclonal antibody using free flow electrophoresis fractionation and mass spectrometry.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113217
Abstract: Antibody charge heterogeneity is one of the major product-related variants in recombinant biopharmaceuticals, which has been commonly monitored by imaged capillary isoelectric focusing (icIEF). Due to the challenges with sample recovery and fractionation, other charge-based… read more here.
Keywords: fractionation; antibody; flow electrophoresis; free flow ... See more keywords
Nonaqueous Micro Free-Flow Electrophoresis for Continuous Separation of Reaction Mixtures in Organic Media.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b00714
Abstract: The continuous separation mechanism of micro free-flow electrophoresis (μFFE) is a straightforward, suitable tool for microscale purification of reaction mixtures. However, aqueous separation buffers and organic reaction solvents limit the applicability of this promising combination.… read more here.
Keywords: separation; flow electrophoresis; free flow; reaction ... See more keywords