Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein.
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DOI: 10.1007/978-1-4939-6875-6_9
Abstract: In this chapter we describe the use of stopped flow fluorescence spectroscopy to analyze the kinetic mechanisms of protein mediated cholesterol transfer to, from, and between model membranes. These assays allow for the detection of… read more here.
Keywords: stopped flow; flow fluorescence; transport; cholesterol ... See more keywords