Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2217-9_3
Abstract: Multiparameter fluorescence-activated cell sorting (FACS) procedure separates target cells from a total population of cells by using specific signatures that the target cell expresses on their cell surface. For human lymphatic endothelial cells (LECs) this… read more here.
Keywords: activated cell; fluorescence activated; cell sorting; cell ... See more keywords
Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7428-3_9
Abstract: To study the process of erythropoiesis, it is important to be able to isolate erythroid progenitors and erythroblasts at distinct stages of development. During the past decade, considerable progress has been made on the development… read more here.
Keywords: analysis; erythroid cells; cytometry fcm; flow cytometry ... See more keywords
Fluorescence-Activated Cell Sorting of Murine Mammary Cancer Stem-Like Cell Subpopulations with HIF Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7665-2_22
Abstract: Fluorescence-activated cell sorting (FACS) is a common method to identify and to isolate subpopulations within a complex mixture of cells based on their light scatter and fluorescent staining profiles. FACS is widely used to enrich… read more here.
Keywords: activated cell; cell sorting; fluorescence activated; cancer ... See more keywords
Identifying Differentially Expressed Genes Using Fluorescence-Activated Cell Sorting (FACS) and RNA Sequencing from Low Input Samples.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8618-7_6
Abstract: Cell type-specific gene expression profiles are useful for understanding genes that are important for the development of different tissues and organs. Here, we describe how to perform fluorescence-activated cell sorting (FACS) on Arabidopsis root protoplasts… read more here.
Keywords: activated cell; cell sorting; sorting facs; rna sequencing ... See more keywords
Isolation of Tailor-Made Antibody Fragments from Yeast-Displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_14
Abstract: In the past decades, monoclonal antibodies have made an unprecedented transformation from research tools to a rapidly growing class of therapeutics. Advancements in the yeast surface display platform enable the selection of favorable mouse or… read more here.
Keywords: activated cell; antibody; selection; cell receptor ... See more keywords
Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_15
Abstract: Anti-hapten antibodies are widely used as specific immunochemical detection tools in a variety of clinical and environmental analyses. The sensitivity, however, is limited due to the resulting antibody affinities to the haptens which, in turn,… read more here.
Keywords: activated cell; hapten antibodies; cell sorting; fluorescence activated ... See more keywords
Engineering Riboswitches in Vivo Using Dual Genetic Selection and Fluorescence-Activated Cell Sorting.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00099
Abstract: Riboswitches, noncoding RNAs that bind a small molecule effector to control gene expression at the level of transcription or translation, are uniquely suited to meet challenges in synthetic biology. To expand the limited set of… read more here.
Keywords: activated cell; genetic selection; selection fluorescence; biology ... See more keywords
Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00103
Abstract: Synthetic biology aims to improve human health and the environment by repurposing biological enzymes for use in practical applications. However, natural enzymes often function with suboptimal activity when engineered into biological pathways or challenged to… read more here.
Keywords: droplet sorting; biology; activated droplet; directed evolution ... See more keywords
A Modified Method for Purifying Gallbladder Epithelial Cells Using Fluorescence-activated Cell Sorting.
Sign Up to like & getrecommendations! Published in 2017 at "In vivo"
DOI: 10.21873/invivo.11041
Abstract: BACKGROUND We have previously reported a procedure for isolating and culturing biliary epithelial cells (BECs). The aim of this study was to reconsider the method for obtaining pure BECs using the mouse gallbladder. MATERIALS AND… read more here.
Keywords: pure becs; epithelial cells; activated cell; becs ... See more keywords
Fluorescence activated cell sorting and fermentation analysis to study rumen microbiome responses to administered live microbials and yeast cell wall derived prebiotics
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1020250
Abstract: Rapid dietary changes, such as switching from high-forage to high-grain diets, can modify the rumen microbiome and initiate gastrointestinal distress, such as bloating. In such cases, feed additives, including prebiotics and live microbials, can be… read more here.
Keywords: rumen microbiome; significant changes; bio mos; live microbials ... See more keywords
Rapid Identification of Functional Pyrrolysyl-tRNA Synthetases via Fluorescence-Activated Cell Sorting
Sign Up to like & getrecommendations! Published in 2018 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20010029
Abstract: The orthogonal pyrrolysyl-tRNA synthetase/tRNACUA pair and their variants have provided powerful tools for expanding the genetic code to allow for engineering of proteins with augmented structure and function not present in Nature. To expedite the… read more here.
Keywords: pyrrolysyl trna; pyrrolysyl; activated cell; cell sorting ... See more keywords