Disruptive effect of tocopherol oxalate on DPPC liposome structure: DSC, SAXS, and fluorescence anisotropy studies.
Sign Up to like & getrecommendations! Published in 2018 at "Chemistry and physics of lipids"
DOI: 10.1016/j.chemphyslip.2018.10.001
Abstract: α-Tocopherol oxalate (TO), a tocopherol ester derivative, was investigated for its effect on the structural changes of fully hydrated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes, as a function of concentration and temperature, by applying differential scanning calorimetry (DSC),… read more here.
Keywords: phase; fluorescence anisotropy; tocopherol oxalate; anisotropy ... See more keywords
Directing a rational design of aptamer-based fluorescence anisotropy assay for sensitive detection of immunoglobulin E by site-specific binding study.
Sign Up to like & getrecommendations! Published in 2020 at "Talanta"
DOI: 10.1016/j.talanta.2020.121018
Abstract: Mapping aptamer-protein interactions is important for characterization and applications of aptamers against proteins. We describe here probing affinity interactions between aptamer and immunoglobulin E (IgE) with a fluorescence anisotropy (FA) approach using a series of… read more here.
Keywords: affinity; fluorescence anisotropy; rational design; site ... See more keywords
Fluorescence Anisotropy as a Self-Referencing Readout for Ion-Selective Sensing and Imaging Using Homo-FRET between Chromoionophores.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01532
Abstract: Fluorescence anisotropy has been widely used in developing biosensors and immunoassays, by virtue of the self-reference and environment-sensitive properties. However, fluorescence anisotropic chemical sensors on inorganic ions are limited by the total anisotropy change. To… read more here.
Keywords: fluorescence anisotropy; homo fret; fluorescence; ion selective ... See more keywords
Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets
Sign Up to like & getrecommendations! Published in 2017 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.6b02528
Abstract: Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplets are shown to yield high-resolution binding curves from which precise dissociation constants (Kd) for protein–peptide interactions can be inferred. With the current platform, four titrations… read more here.
Keywords: fluorescence; fluorescence anisotropy; anisotropy measurements; individual nanoliter ... See more keywords
5-Carboxytetramethylrhodamine-Ampicillin Fluorescence Anisotropy-Based Assay of Escherichia coli Penicillin-Binding Protein 2 Transpeptidase Inhibition.
Sign Up to like & getrecommendations! Published in 2019 at "ACS infectious diseases"
DOI: 10.1021/acsinfecdis.8b00351
Abstract: The high-molecular mass penicillin-binding proteins (PBPs) are the essential targets of the β-lactam classes of antibacterial drugs. In the Gram-negative pathogen Escherichia coli, these include PBP1a, PBP1b, PBP2, and PBP3. Techniques that enable facile measurement… read more here.
Keywords: ampicillin; fluorescence; fluorescence anisotropy; inhibition ... See more keywords
Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d1ra08977c
Abstract: We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases… read more here.
Keywords: temperature; pressure; fluorescence anisotropy; pressure temperature ... See more keywords
Fluorescence anisotropy using highly polarized emitting dyes confined inside BNNTs.
Sign Up to like & getrecommendations! Published in 2023 at "Materials horizons"
DOI: 10.1039/d2mh01239a
Abstract: Polarized fluorescence emission of nanoscale emitters has been extensively studied for applications such as bioimaging, displays, and optical communication. Extending the polarization properties in large assemblies of compact emitters is, however, challenging because of self-aggregation… read more here.
Keywords: anisotropy using; fluorescence anisotropy; confined inside; using highly ... See more keywords
Fluorescence anisotropy studies on the Hoechst 33258-DNA interaction: the solvent effect
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biomolecular Structure and Dynamics"
DOI: 10.1080/07391102.2020.1782267
Abstract: Abstract Fluorescence anisotropy method was applied to characterize the interactions of DNA minor groove binder Hoechst 33258 with different solvents without and in the presence of DNA. It is important to study the interaction of… read more here.
Keywords: dna; hoechst 33258; fluorescence anisotropy; anisotropy studies ... See more keywords
Imaging the rotational mobility of carbon dot-gold nanoparticle conjugates using frequency domain wide-field time-resolved fluorescence anisotropy
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Biomedical Optics"
DOI: 10.1117/1.jbo.28.5.056001
Abstract: Abstract. Significance Wide-field measurements of time-resolved fluorescence anisotropy (TR-FA) provide pixel-by-pixel information about the rotational mobility of fluorophores, reflecting changes in the local microviscosity and other factors influencing the fluorophore’s diffusional motion. These features offer… read more here.
Keywords: rotational mobility; fluorescence anisotropy; resolved fluorescence; wide field ... See more keywords
Application of a Fluorescence Anisotropy-Based Assay to Quantify Transglutaminase 2 Activity in Cell Lysates
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23094475
Abstract: Transglutaminase 2 (TGase 2) is a multifunctional protein which is involved in various physiological and pathophysiological processes. The latter also include its participation in the development and progression of malignant neoplasms, which are often accompanied… read more here.
Keywords: cell; tgase; fluorescence anisotropy; activity ... See more keywords
Probing Red Blood Cell Membrane Microviscosity Using Fluorescence Anisotropy Decay Curves of the Lipophilic Dye PKH26
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232415767
Abstract: Red blood cell (RBC) aggregation and deformation are governed by the molecular processes occurring on the membrane. Since several social important diseases are accompanied by alterations in RBC aggregation and deformability, it is important to… read more here.
Keywords: fluorescence anisotropy; fluorescence; membrane microviscosity; membrane ... See more keywords