An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation
Sign Up to like & getrecommendations! Published in 2020 at "Chemcatchem"
DOI: 10.1002/cctc.201901891
Abstract: Halide assays are important for the study of enzymatic dehalogenation, a topic of great industrial and scientific importance. Here we describe the development of a very sensitive halide assay that can detect less than a… read more here.
Keywords: dehalogenation; assay detection; ultrasensitive fluorescence; enzymatic dehalogenation ... See more keywords
A turn-on fluorescence assay of alkaline phosphatase activity based on an enzyme-triggered conformational switch of G-quadruplex.
Sign Up to like & getrecommendations! Published in 2020 at "Talanta"
DOI: 10.1016/j.talanta.2019.120453
Abstract: Herein, a turn-on fluorescence assay was introduced for alkaline phosphatase (ALP) detection based on ThT/G-quadruplex system. The basis of the method is that chelation of guanine bases at the binding sites by Ag+ blocks G-quadruplex… read more here.
Keywords: fluorescence; turn fluorescence; fluorescence assay; activity ... See more keywords
DNAzyme and rGO based fluorescence assay for Fpg activity analysis, drug screening, and bacterial imaging.
Sign Up to like & getrecommendations! Published in 2020 at "Talanta"
DOI: 10.1016/j.talanta.2020.121158
Abstract: Due to the significant role of formamidopyrimidine DNA glycosylase (Fpg) in physiological processes and DNA oxidative damage-related diseases, it is essential to establish sensitive methods for monitoring the Fpg activity in vitro and in vivo… read more here.
Keywords: dnazyme rgo; fluorescence; fpg; fluorescence assay ... See more keywords
Cationic Carbon Dots for Modification-Free Detection of Hyaluronidase via an Electrostatic-Controlled Ratiometric Fluorescence Assay.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b01705
Abstract: Carbon dots (CDs) emerge as excellent fluorescent nanomaterials, but the full exploitation and application of their exceptional properties in the development of fluorescence assay are still rare. In this work, cationic carbon dots (C-CDs) covered… read more here.
Keywords: detection; cationic carbon; carbon; carbon dots ... See more keywords
Versatile High-Throughput Fluorescence Assay for Monitoring Cas9 Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01155
Abstract: The RNA-guided DNA nuclease Cas9 is now widely used for the targeted modification of genomes of human cells and various organisms. Despite the extensive use of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) systems for genome… read more here.
Keywords: activity; high throughput; fluorescence assay; cas9 ... See more keywords
An efficient target-intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc04104g
Abstract: An ultrasensitive fluorescence assay for intracellular Pb2+ determination was proposed through target-intermediate recycling amplification based on metal-assisted DNAzyme catalysis and strand displacement reactions. Compared with only target recycling-based fluorescence assay with an M amplification ratio,… read more here.
Keywords: fluorescence; target; ultrasensitive fluorescence; intermediate recycling ... See more keywords
A label-free and sensitive fluorescence assay for hyaluronidase activity through electrostatic-controlled quantum dots self-assembly
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Chemical Research"
DOI: 10.1177/17475198211018973
Abstract: A label-free fluorescence assay for hyaluronidase (HAase) activity based on self-assembly of quantum dots is developed. A cationic polymer (polycation) can induce aggregation of the negatively charged quantum dots through electrostatic interactions and the fluorescence… read more here.
Keywords: fluorescence; assay hyaluronidase; fluorescence assay; label free ... See more keywords
Sensitive and Specific Detection of Lumpy Skin Disease Virus in Cattle by CRISPR-Cas12a Fluorescent Assay Coupled with Recombinase Polymerase Amplification
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13050734
Abstract: Lumpy skin disease (LSD) is a severe and highly infectious pox disease of cattle caused by the lumpy skin disease virus (LSDV). To facilitate early control of LSD, this study aimed to develop a new… read more here.
Keywords: skin disease; lumpy skin; disease; fluorescence assay ... See more keywords
Sensitive, Selective and Reliable Detection of Fe3+ in Lake Water via Carbon Dots-Based Fluorescence Assay
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27196749
Abstract: In this study, C-dots were facilely synthesized via microwave irradiation using citric acid and ethylenediamine as carbon precursors. The fluorescence emissions of the C-dots could be selectively quenched by Fe3+, and the degree of quenching… read more here.
Keywords: carbon; water; fluorescence assay; lake water ... See more keywords
Carbon Dots-Based Fluorescence Assay for the Facile and Reliable Detection of Ag+ in Natural Water and Serum Samples
Sign Up to like & getrecommendations! Published in 2023 at "Molecules"
DOI: 10.3390/molecules28041566
Abstract: In this report, red-emissive carbon dots (C-dots) were facilely prepared from o-phenylenediamine via microwave-assisted hydrothermal treatment. The C-dots demonstrated excitation wavelength-independent emission with maximums at 621 nm that could be effectively quenched by Ag+ via… read more here.
Keywords: water; fluorescence assay; detection; carbon dots ... See more keywords