Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point-of-Care: A Review of Recent Technologies.
Sign Up to like & getrecommendations! Published in 2022 at "Advanced materials"
DOI: 10.1002/adma.202107986
Abstract: Fluorescence-based biosensors have widely been used in the life-sciences and biomedical applications due to their low limit of detection and a diverse selection of fluorophores that enable simultaneous measurements of multiple biomarkers. Recent research effort… read more here.
Keywords: point care; fluorescence enhancement; fluorescence based; metal ... See more keywords
Boron-Conjugated Pyrenes as Fluorescence-Based Molecular Probes and Security Markers.
Sign Up to like & getrecommendations! Published in 2019 at "ChemPlusChem"
DOI: 10.1002/cplu.201900280
Abstract: Boron-embedded aromatic hydrocarbons are a class of molecules known for their distinct electronic and/or optoelectronic properties and are thus suitable for many potential applications. Among those, boronic ester and acid containing molecules have been widely… read more here.
Keywords: pyrenes fluorescence; fluorescence based; fluorescence; boron conjugated ... See more keywords
5‐Hydroxymethylcytosine as a clinical biomarker: Fluorescence‐based assay for high‐throughput epigenetic quantification in human tissues
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Cancer"
DOI: 10.1002/ijc.32519
Abstract: Epigenetic transformations may provide early indicators for cancer and other disease. Specifically, the amount of genomic 5‐hydroxymethylcytosine (5‐hmC) was shown to be globally reduced in a wide range of cancers. The integration of this global… read more here.
Keywords: biomarker; fluorescence based; cancer; quantification ... See more keywords
A Fluorescence-Based Assay for Quantitative Analysis of Phospholipid:Diacylglycerol Acyltransferase Activity.
Sign Up to like & getrecommendations! Published in 2019 at "Lipids"
DOI: 10.1002/lipd.12190
Abstract: Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl-CoA-independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled… read more here.
Keywords: phospholipid diacylglycerol; fluorescence based; diacylglycerol; diacylglycerol acyltransferase ... See more keywords
Improving the Sensitivity of Fluorescence-Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads.
Sign Up to like & getrecommendations! Published in 2019 at "Small"
DOI: 10.1002/smll.201803751
Abstract: In fluorescence-based assays, usually a target molecule is captured using a probe conjugated to a capture surface, and then detected using a second fluorescently labeled probe. One of the most common capture surfaces is a… read more here.
Keywords: fluorescence based; magnetic beads; sensitivity; autofluorescence ... See more keywords
In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0219-5_7
Abstract: Proper cell division and the equal segregation of genetic material are essential for life. Cell division is mediated by the mitotic spindle, which is composed of microtubules (MTs) and MT-associated proteins that help align and… read more here.
Keywords: protein; protein interactions; fret fluorescence; fluorescence based ... See more keywords
A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1166-1_8
Abstract: Postsynaptic density-95, disks-large, and zonula occludens-1 (PDZ) domain interactions with cognate linear binding motifs (i.e., PDZ-binding motifs or PBMs) are important for many biological processes and can be pathological when disrupted. There are hundreds of… read more here.
Keywords: thermodynamics; fluorescence based; assay determine; pdz pbm ... See more keywords
Fluorescence-Based Assay For Measuring OMA1 Activity.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1266-8_24
Abstract: Mitochondrial fusion depends on proteolytic processing of the dynamin-related GTPase protein, OPA1, which is regulated by the mitochondrial zinc metalloproteinase, OMA1. Last year we published a report describing a novel approach to directly measure the… read more here.
Keywords: fluorescence based; oma1 activity; assay measuring; based assay ... See more keywords
Fluorescence-Based Real-Time Activity Assays to Identify RNase P Inhibitors.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6634-9_12
Abstract: Transfer RNA is transcribed as precursor molecules that are processed before participating in translation catalyzed by the ribosome. Ribonuclease P is the endonuclease that catalyzes the 5' end maturation of precursor tRNA and it is… read more here.
Keywords: real time; fluorescence based; rnase; based real ... See more keywords
Gauging and Visualizing c-di-GMP Levels in Pseudomonas aeruginosa Using Fluorescence-Based Biosensors.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7240-1_8
Abstract: Recent research has shown that the molecule c-di-GMP is an important second messenger regulating various functions in bacteria. In particular, the implication of c-di-GMP as a positive regulator of adhesion and biofilm formation has gained… read more here.
Keywords: pseudomonas aeruginosa; gmp levels; fluorescence based; gmp ... See more keywords
Fluorescence-Based Methods for Characterizing RNA Interactions In Vivo.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7634-8_9
Abstract: Fluorescence-based tools that measure RNA-RNA and RNA-protein interactions in vivo offer useful experimental approaches to probe the complex and dynamic physiological behavior of bacterial RNAs. Here we document the step-by-step design and application of two… read more here.
Keywords: methods characterizing; fluorescence; interactions vivo; fluorescence based ... See more keywords