Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_5
Abstract: Examining protein-protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize intracellular protein-protein interactions and ascertain… read more here.
Keywords: protein interactions; protein protein; fluorescence complementation; protein ... See more keywords
Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8820-4_11
Abstract: Neuronal accumulations of the presynaptic protein α-synuclein represent a hallmark of Parkinson's disease (PD). Multiple system atrophy and dementia with Lewy bodies are other neurodegenerative diseases in which α-synuclein aggregates are the main pathological characteristic.… read more here.
Keywords: protein; fluorescence complementation; aggregation; bimolecular fluorescence ... See more keywords
Trimolecular Fluorescence Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in Plants.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9045-0_19
Abstract: RNA-protein interactions play important roles in various eukaryotic biological processes. Molecular imaging of subcellular localization of RNA-protein complexes in plants is critical for understanding these interactions. However, methods to image RNA-protein interactions in living plants… read more here.
Keywords: protein interaction; fluorescence complementation; rna; rna protein ... See more keywords
A tandem near-infrared fluorescence complementation system with enhanced fluorescence for imaging protein-protein interactions in vivo.
Sign Up to like & getrecommendations! Published in 2020 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2020.120544
Abstract: Bimolecular fluorescence complementation (BiFC) is an effective tool for visualizing protein-protein interactions (PPIs). However, a BiFC system with long wavelength and high fluorescence intensity is yet to be developed for in vivo imaging. In this… read more here.
Keywords: protein; ppis; system; fluorescence ... See more keywords
A Bi-fluorescence complementation system to detect associations between the Endoplasmic reticulum and mitochondria
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-17278-1
Abstract: Close contacts between the endoplasmic reticulum membrane and the mitochondrial outer membrane facilitate efficient transfer of lipids between the organelles and coordinate Ca2+ signalling and stress responses. Changes to this coupling is associated with a… read more here.
Keywords: endoplasmic reticulum; reticulum; fluorescence complementation; complementation system ... See more keywords
Biomolecular Fluorescence Complementation Profiling and Artificial Intelligence Structure Prediction of the Kaposi’s Sarcoma-Associated Herpesvirus ORF18 and ORF30 Interaction
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23179647
Abstract: Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi’s sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman’s disease. During KSHV lytic infection, lytic-related genes, categorized as immediate-early, early, and late genes, are expressed in… read more here.
Keywords: orf18 orf30; interaction; kaposi sarcoma; associated herpesvirus ... See more keywords