Articles with "fluorescence detection" as a keyword



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Electrokinetic characterization of hybrid NOA 81‐glass microchips: Application to protein microchip electrophoresis with indirect fluorescence detection

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Published in 2022 at "ELECTROPHORESIS"

DOI: 10.1002/elps.202200057

Abstract: A low‐cost and straightforward hybrid NOA (Norland optical adhesive) 81‐glass microchip electrophoresis device was designed and developed for protein separation using indirect fluorescence detection. This new microchip was first characterized in terms of surface charge… read more here.

Keywords: glass; indirect fluorescence; fluorescence detection; electrophoresis ... See more keywords
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Separation of ofloxacin enantiomers by CE with fluorescence detection using DNA oligonucleotides as chiral selectors.

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Published in 2022 at "Journal of separation science"

DOI: 10.1002/jssc.202200209

Abstract: This study used CE with fluorescence detection- and partial-filling mode-based method for chiral separation of ofloxacin. The DNA oligonucleotides with different base sequences were studied as potential chiral selectors including DNA tetrahedron, G-quadruplex, and G-riched… read more here.

Keywords: fluorescence detection; dna; chiral selectors; separation ofloxacin ... See more keywords
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High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.

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Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6421-5_8

Abstract: Recent advances in optical tweezers have greatly expanded their measurement capabilities. A new generation of hybrid instrument that combines nanomechanical manipulation with fluorescence detection-fluorescence optical tweezers, or "fleezers"-is providing a powerful approach to study complex… read more here.

Keywords: optical tweezers; high resolution; single molecule; molecule fluorescence ... See more keywords
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Aggregation Profiling of C9orf72 Dipeptide Repeat Proteins Transgenically Expressed in Drosophila melanogaster Using an Analytical Ultracentrifuge Equipped with Fluorescence Detection.

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Published in 2019 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-9678-0_6

Abstract: The recent development of a fluorescence detection system for the analytical ultracentrifuge has allowed for the characterization of protein size and aggregation in complex mixtures. Protocols are described here to analyze protein aggregation seen in… read more here.

Keywords: analytical ultracentrifuge; sedimentation velocity; aggregation; fluorescence detection ... See more keywords
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Carbon dots incorporated metal–organic framework for enhancing fluorescence detection performance

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Published in 2020 at "Journal of Materials Science"

DOI: 10.1007/s10853-020-05027-1

Abstract: The fluorescence detection of environmental pollution residues is hugely significant; thus, the improvements in sensitivity and selectivity are still a great task. In this work, a novel molecularly imprinted fluorescence sensor (CDs&ZIF-8@MIPs) with a core–shell… read more here.

Keywords: detection; fluorescence; carbon dots; selectivity ... See more keywords
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Determination of Biogenic Amines in Fish Meat and Fermented Foods Using Column-Switching High-Performance Liquid Chromatography with Fluorescence Detection

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Published in 2018 at "Food Analytical Methods"

DOI: 10.1007/s12161-018-1349-0

Abstract: In this study, we developed a column-switching high-performance liquid chromatography method with fluorescence detection for the simultaneous analysis of biogenic amines (BAs). This method uses an isocratic solution using acetonitrile with water as the mobile… read more here.

Keywords: switching high; column switching; fluorescence detection;
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Flavonoid moiety-incorporated carbon dots for ultrasensitive and highly selective fluorescence detection and removal of Pb2+

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Published in 2018 at "Nano Research"

DOI: 10.1007/s12274-017-1931-6

Abstract: A new type of self-targeting carbon dot (CD-Fla) for the detection of the toxic heavy metal ion Pb2+ was synthesized via a one-pot hydrothermal route using flavonoid extracts of Ginkgo biloba leaves as the starting… read more here.

Keywords: pb2; fluorescence; detection removal; removal pb2 ... See more keywords
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A radiation tolerant laser-induced fluorescence detection system for a potential Europa Lander mission

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Published in 2021 at "Acta Astronautica"

DOI: 10.1016/j.actaastro.2021.06.012

Abstract: Abstract Analytical techniques commonly implemented for in situ organic analysis on space missions lack the sensitivity or specificity necessary to definitively characterize key markers of current or past life, such as amino acids. To this… read more here.

Keywords: system; laser; fluorescence detection; laser induced ... See more keywords
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Fluorescence detection and identification of eight sulphonamides using capillary electrophoresis on released excipients in lake water

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Published in 2019 at "Arabian Journal of Chemistry"

DOI: 10.1016/j.arabjc.2014.10.039

Abstract: Abstract A simple and sensitive capillary electrophoresis method with fluorescence detection was developed for the determination of sulphanilamide, sulphamerazine, sulphacetamide and sulphanilic acid, sulphathiazole, Sulphisomidine, sulphadoxine and sulphadiazine in lake water. The sulphonamides were extracted… read more here.

Keywords: lake water; capillary electrophoresis; eight sulphonamides; fluorescence detection ... See more keywords
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The determination of pharmaceutically active thiols using hydrophilic interaction chromatography followed postcolumn derivatization with o‐phthaldialdehyde and fluorescence detection

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Published in 2018 at "Journal of Pharmaceutical and Biomedical Analysis"

DOI: 10.1016/j.jpba.2018.04.007

Abstract: Graphical abstract Figure. No caption available. HighlightsA novel HILIC method for the determination of pharmaceutically active thiols was presented.The effect of chromatographic conditions on the retention was investigated.The postcolumn reaction conditions such as reaction temperature… read more here.

Keywords: determination; postcolumn derivatization; active thiols; pharmaceutically active ... See more keywords
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Liquid chromatography method with tandem mass spectrometry and fluorescence detection for determination of inflammatory biomarkers in gingival crevicular fluid as a tool for diagnosis of periodontal disease.

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Published in 2022 at "Journal of pharmaceutical and biomedical analysis"

DOI: 10.1016/j.jpba.2022.114644

Abstract: The new ultra-high performance liquid chromatography method with tandem mass spectrometry and fluorescence detection allowing fast, selective, and high-throughput analysis of neopterin, kynurenine, tryptophan, and creatinine in gingival crevicular fluid (GCF) has been optimized. Defining… read more here.

Keywords: mass spectrometry; fluorescence detection; periodontal disease; method ... See more keywords