Increasing fluorescence lifetime for resolution improvement in stimulated emission depletion nanoscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biophotonics"
DOI: 10.1002/jbio.201800315
Abstract: Super-resolution microscopy (SRM) has had a substantial impact on the biological sciences due to its ability to observe tiny objects less than 200 nm in size. Stimulated emission depletion (STED) microscopy represents a major category of… read more here.
Keywords: resolution; microscopy; fluorescence; depletion ... See more keywords
Fluorescence lifetime metabolic mapping of hypoxia-induced damage in pancreatic pseudo-islets.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of biophotonics"
DOI: 10.1002/jbio.202000375
Abstract: Pancreatic islet isolation from donor pancreases is an essential step for the transplantation of insulin-secreting β-cells as a therapy to treat type 1 diabetes mellitus. This process however damages islet basement membranes, which can lead… read more here.
Keywords: islet; pseudo islets; transplantation; lifetime metabolic ... See more keywords
Quenched coumarin derivatives as fluorescence lifetime phantoms for NADH and FAD†.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of biophotonics"
DOI: 10.1002/jbio.202100024
Abstract: 2-photon fluorescence lifetime imaging is a versatile laboratory technique in the field of biophotonics and its importance is also growing in the field of in vivo diagnostics for medical purposes. After years of experience in… read more here.
Keywords: reference samples; fluorescence; quenched coumarin; nadh fad ... See more keywords
Intraoperative detection of IDH‐mutant glioma using fluorescence lifetime imaging
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Biophotonics"
DOI: 10.1002/jbio.202200291
Abstract: Identifying isocitrate dehydrogenase (IDH)‐mutation and glioma subtype during surgery instead of days later can aid in modifying tumor resection strategies for better survival outcomes. We report intraoperative identification of IDH‐mutant glioma (N = 12 patients)… read more here.
Keywords: idh mutant; mutant glioma; glioma; fluorescence lifetime ... See more keywords
Super-Resolved FRET Imaging by Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Small methods"
DOI: 10.1002/smtd.202201565
Abstract: Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions… read more here.
Keywords: super resolved; resolved fret; microscopy; fluorescence lifetime ... See more keywords
Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_4
Abstract: Caveolae are bulb-shaped invaginations of the plasma membrane that are enriched in specific lipids including cholesterol, phosphatidylserine and sphingolipids. Caveolae have many described cellular roles and functions, including endocytic transport, transcytosis, mechanosensing, and serving as… read more here.
Keywords: microscopy; protein interactions; fluorescence lifetime; membrane ... See more keywords
In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer Through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM).
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7262-3_14
Abstract: Combinations of multiple fluorescent fusion proteins are commonly generated and used for colocalization studies in live cell imaging but also biochemical analysis of protein-protein interactions by co-immunoprecipitation in vitro. Advanced microscopy techniques like Förster resonance… read more here.
Keywords: fret flim; energy transfer; microscopy; interaction ... See more keywords
Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM).
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7856-4_10
Abstract: Anthocyanins are intrinsically fluorescent pigments that accumulate in plant vacuoles. We have developed a platform to analyze the fluorescence decay of anthocyanins by fluorescence lifetime imaging microscopy (FLIM), under in vitro and in vivo conditions.… read more here.
Keywords: microscopy flim; fluorescence; microscopy; anthocyanins fluorescence ... See more keywords
Fluorescence Lifetime Imaging of Plant Cell Walls.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9469-4_5
Abstract: Fluorescence is a versatile property of many molecules called fluorophores. In plant cell walls, fluorescence is generally attributed to aromatic molecules such as lignin. In contrary to fluorescence intensity, fluorescence lifetime is independent from fluorophore… read more here.
Keywords: fluorescence; cell walls; fluorescence lifetime; cell ... See more keywords
Monitoring Macromolecular Crowding via FRET using Wavelength-Dependent Fluorescence Lifetime Measurements
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1885
Abstract: Macromolecular crowding influences many intracellular processes such as protein folding, biochemical reactions and diffusion. Recently, a rationally designed FRET probe was developed to quantify macromolecular crowding in vitro (Boersma et al. Nat. Meth. 12:227, 2015).… read more here.
Keywords: monitoring macromolecular; fluorescence lifetime; macromolecular crowding; lifetime measurements ... See more keywords
Peripapillary fluorescence lifetime reveals age‐dependent changes using fluorescence lifetime imaging ophthalmoscopy in rats
Sign Up to like & getrecommendations! Published in 2018 at "Experimental Eye Research"
DOI: 10.1016/j.exer.2018.07.008
Abstract: &NA; Many fundus diseases accompany fundus autofluorescence change. Fluorescence lifetime imaging ophthalmoscope (FLIO) is a latest technique in imaging fundus autofluorescence. With FLIO, the fundus fluorescence lifetime (FLT) is recorded topographically, assisting to diagnose and… read more here.
Keywords: fluorescence; age; fluorescence lifetime; fundus ... See more keywords