Cardiac troponin and tropomyosin bind to F‐actin cooperatively, as revealed by fluorescence microscopy
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12876
Abstract: In cardiac muscle, binding of troponin (Tn) and tropomyosin (Tpm) to filamentous (F)‐actin forms thin filaments capable of Ca2+‐dependent regulation of contraction. Tpm binds to F‐actin in a head‐to‐tail fashion, while Tn stabilizes these linkages.… read more here.
Keywords: tpm; microscopy; actin; fluorescence microscopy ... See more keywords
Monitoring Sphingolipid Trafficking in Cells using Fluorescence Microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.67
Abstract: Sphingolipids are structural components of organelle membranes that also participate in signal transduction pathways. Complex sphingolipids are trafficked from their site of synthesis in organelles of the early secretory pathway to the Golgi apparatus, the… read more here.
Keywords: microscopy; sphingolipid trafficking; fluorescence microscopy; complex sphingolipids ... See more keywords
Object‐Oriented Segmentation of Cell Nuclei in Fluorescence Microscopy Images
Sign Up to like & getrecommendations! Published in 2018 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23594
Abstract: Cell nucleus segmentation remains an open and challenging problem especially to segment nuclei in cell clumps. Splitting a cell clump would be straightforward if the gradients of boundary pixels in‐between the nuclei were always higher… read more here.
Keywords: microscopy; segmentation; fluorescence microscopy; level ... See more keywords
Rigor and Reproducibility in Confocal Fluorescence Microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23924
Abstract: Fluorescence microscopy can be an excellent tool for making quantitative measurements in cells. For particularly rigorous applications, many choose to use confocal microscopes, which use pinholes to throw away the out-of-focus signal, producing an image… read more here.
Keywords: reproducibility confocal; microscopy; fluorescence microscopy; rigor reproducibility ... See more keywords
Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods.
Sign Up to like & getrecommendations! Published in 2023 at "European journal of immunology"
DOI: 10.1002/eji.202249923
Abstract: This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and… read more here.
Keywords: microscopy; multiparameter fluorescence; analysis; microscopy imaging ... See more keywords
Illuminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction
Sign Up to like & getrecommendations! Published in 2025 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.70046
Abstract: In this letter, we put forward the light sheet fluorescence microscopy (LSFM) as a cutting‐edge tool for 3D imaging of whole skeletal muscle, focusing on satellite cells (SCs). SCs represent the resident adult muscle stem… read more here.
Keywords: forced eccentric; satellite cells; sheet fluorescence; fluorescence microscopy ... See more keywords
Imaging the Deep Spinal Cord Microvascular Structure and Function with High‐Speed NIR‐II Fluorescence Microscopy (Small Methods 8/2022)
Sign Up to like & getrecommendations! Published in 2022 at "Small Methods"
DOI: 10.1002/smtd.202270047
Abstract: The spinal cord (SC) is crucial for a myriad of somatosensory, autonomic signal processing, and transductions. Understanding the SC vascular structure and function thus plays an integral part in neuroscience and clinical research. However, the… read more here.
Keywords: speed; nir fluorescence; microscopy; spinal cord ... See more keywords
Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms: A Fluorescence Microscopy Approach.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0896-8_11
Abstract: Reactive oxygen species (ROS) production within biofilms is studied with a simple and easy setup based on fluorescence microscopy. Herein, a biofilm is exposed to different ROS inducers: a bactericidal antibiotic (ciprofloxacin) and a BODIPY-based… read more here.
Keywords: ros production; fluorescence microscopy; revealing ros; microscopy ... See more keywords
Measuring Phagosomal pH by Fluorescence Microscopy.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6581-6_12
Abstract: Dual wavelength ratiometric imaging has become a powerful tool for the study of pH in intracellular compartments. It allows for the dynamic imaging of live cells while accounting for changes in the focal plane, differential… read more here.
Keywords: microscopy; fluorescence microscopy; measuring phagosomal; phagosomal fluorescence ... See more keywords
Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7271-5_8
Abstract: Transportation of organelles and biomolecules is vital for many cellular processes. Single-molecule (SM) fluorescence microscopy can expose molecular aspects of the dynamics that remain unresolved in ensemble experiments. For example, trajectories of individual, moving biomolecules… read more here.
Keywords: molecule fluorescence; single molecule; fluorescence microscopy; microscopy ... See more keywords
Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7546-4_10
Abstract: Cellular structures and biomolecular complexes are not simply assemblies of proteins, but are organized with defined numbers of protein molecules in precise locations. Thus, evaluating the spatial localization and numbers of protein molecules is of… read more here.
Keywords: gfp nucleoporin; microscopy; fluorescence microscopy; estimation gfp ... See more keywords