Ligand Discovery: High-Throughput Binding: Fluorescence Polarization (Anisotropy).
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1197-5_10
Abstract: High-throughput assays based on fluorescence polarization (or fluorescence anisotropy) technology have often been employed for primary hit-finding in drug discovery. These binding assays provide a homogeneous format and consistent performance and offer advantages over some… read more here.
Keywords: throughput; anisotropy; fluorescence polarization; high throughput ... See more keywords
Potential of fluorescence polarization immunoassay for the detection of Aspergillus fumigatus galactomannan
Sign Up to like & getrecommendations! Published in 2019 at "Russian Chemical Bulletin"
DOI: 10.1007/s11172-019-2713-y
Abstract: Galactomannan (GM) is a specific polysaccharide antigen of opportunistic mold fungi of the genus Aspergillus. The detection of GM in patients’ biological fluids is a reliable criterion for the diagnosis of invasive pulmonary aspergillosis, a… read more here.
Keywords: potential fluorescence; detection; fluorescence polarization; polarization immunoassay ... See more keywords
Fluorescence Polarization Immunoassay for Alternaria Mycotoxin Tenuazonic Acid Detection and Molecular Modeling Studies of Antibody Recognition
Sign Up to like & getrecommendations! Published in 2018 at "Food Analytical Methods"
DOI: 10.1007/s12161-018-1236-8
Abstract: A simple and rapid method of fluorescence polarization immunoassay (FPIA) for detection of Alternaria mycotoxin tenuazonic acid (TeA) was described. In this study, the tracers TeAH-FITC and TeAH-DTAF were synthesized from hapten 5-(sec-butyl)-3-(1-hydrazonoethyl)-4-hydroxy-1H-pyrrol-2(5H)-one (TeAH) and… read more here.
Keywords: detection; fluorescence polarization; mycotoxin tenuazonic; recognition ... See more keywords
Ligand binding kinetics at histamine H3 receptors by fluorescence‐polarization with real‐time monitoring
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Pharmacology"
DOI: 10.1016/j.ejphar.2019.01.041
Abstract: &NA; Growing evidence recommends incorporating the concept of drug‐target residence times within drug development and screening programs. For many targets, systematic research for binding kinetics is emerging and reported, as in case of the histamine… read more here.
Keywords: binding kinetics; ligand; fluorescence polarization; real time ... See more keywords
Application of fluorescence polarization immunoassay for determination of carbamazepine in wastewater.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of environmental management"
DOI: 10.1016/j.jenvman.2017.01.063
Abstract: Carbamazepine is an antiepileptic drug that can be used as a marker for the cleaning efficiency of wastewater treatment plants. Here, we present the optimization of a fast and easy on-site measurement system based on… read more here.
Keywords: carbamazepine wastewater; wastewater; fluorescence polarization; polarization immunoassay ... See more keywords
Fluorescence polarization immunoassay of colchicine
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2018.07.008
Abstract: Graphical abstract Figure. No caption available. HighlightsFluorescence polarization immunoassay was developed to detect colchicine.Duration of the analysis was 10 min.Detection limit of the analysis was 1.8 ng/mL.Applicability of the assay for control of drug formulations,… read more here.
Keywords: fluorescence polarization; analysis; polarization; colchicine ... See more keywords
Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c02300
Abstract: Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a… read more here.
Keywords: fluorescence polarization; protein determination; noncompetitive fluorescence; immunoassay protein ... See more keywords
Direct Detection of Products from S-Adenosylmethionine-Dependent Enzymes Using a Competitive Fluorescence Polarization Assay.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03556
Abstract: S-Adenosylmethionine (AdoMet)-dependent methyltransferases (MTases) are an essential superfamily of enzymes that catalyze the transfer of a methyl group to several biomolecules. Alterations in the methylation of cellular components crucially impact vital biological processes, making MTases… read more here.
Keywords: dependent enzymes; competitive fluorescence; fluorescence polarization; polarization assay ... See more keywords
Panoply of Fluorescence Polarization/Anisotropy Signaling Mechanisms for Functional Nucleic Acid-Based Sensing Platforms.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b04593
Abstract: Fluorescence polarization/anisotropy is a very popular technique that is widely used in homogeneous-phase immunoassays for the small molecule quantification. In the present Feature, we discuss how the potential of this signaling approach considerably expanded during… read more here.
Keywords: nucleic acid; functional nucleic; fluorescence polarization; polarization anisotropy ... See more keywords
Fluorescence polarization-based rapid detection system for salivary biomarkers using modified DNA aptamers containing base-appended bases.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b03450
Abstract: The field of care testing toward the analysis of blood and saliva, lacks nowadays simple test techniques for biomarkers. In this study, we have developed a novel nucleobase analog, Ugu, which is an uracil derivative… read more here.
Keywords: detection system; base; fluorescence polarization;
Luminescent Polymorphic Co-crystals: A Promising Way to the Diversity of Molecular Assembly, Fluorescence Polarization and Optical Waveguide.
Sign Up to like & getrecommendations! Published in 2020 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.0c06794
Abstract: The design of molecular optoelectronic materials based on fabricating polymorphs and/or co-crystals has been received much recent attention in the fields of luminescence, sensor, nonlinear optics, and so on. If combining the advantages of two… read more here.
Keywords: fluorescence polarization; polarization optical; luminescent polymorphic; optical waveguide ... See more keywords