Articles with "fluorescence situ" as a keyword



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A comprehensive model for the diffusion and hybridization processes of nucleic acid probes in fluorescence in situ hybridization

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Published in 2020 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.27462

Abstract: Fluorescence in situ hybridization (FISH) has been extensively used in the past decades for the detection and localization of microorganisms. However, a mechanistic approach of the whole FISH process is still missing, and the main… read more here.

Keywords: diffusion; fluorescence situ; hybridization; situ hybridization ... See more keywords
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Fluorescence in situ hybridization for ETV6 gene rearrangements on destained cytological smears: Role in diagnosis of secretory carcinoma of salivary gland.

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Published in 2023 at "Cancer cytopathology"

DOI: 10.1002/cncy.22699

Abstract: BACKGROUND Fine-needle aspiration cytology (FNAC) is the first-line diagnostic procedure for salivary gland masses. Secretory carcinoma (SC) is characterized by ETV6 and RET rearrangements detected by fluorescence in situ hybridization (FISH) or reverse transcriptase-polymerase chain… read more here.

Keywords: situ hybridization; diagnosis; fluorescence situ; secretory carcinoma ... See more keywords
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The combined application of urinary liquid‐based cytology with fluorescence in situ hybridization and p16/Ki‐67 dual immunostaining is valuable for improving the early diagnosis of upper tract urothelial carcinomas

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Published in 2017 at "Diagnostic Cytopathology"

DOI: 10.1002/dc.23796

Abstract: To evaluate the diagnostic values of urine liquid‐based cytology (LBC), fluorescence in situ hybridization (FISH), and p16/Ki‐67 dual immunostaining in the detection of upper tract urothelial carcinomas (UTUCs). read more here.

Keywords: p16 dual; fluorescence situ; liquid based; based cytology ... See more keywords
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Diagnostic pitfalls and the value of fluorescence in situ hybridization as an adjunct to cytologic evaluation of bile duct brushings in patients with primary sclerosing cholangitis

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Published in 2022 at "Diagnostic Cytopathology"

DOI: 10.1002/dc.25059

Abstract: Primary sclerosing cholangitis (PSC) can present diagnostic difficulties on bile duct brushings as cytologic features mimicking adenocarcinoma may exist. This study evaluates the role of fluorescence in‐situ hybridization (FISH) as an adjunct to cytologic evaluation. read more here.

Keywords: situ hybridization; sclerosing cholangitis; fluorescence situ; primary sclerosing ... See more keywords
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Quantitative Fluorescence In Situ Hybridization (FISH) of Magnetically Confined Bacteria Enables Early Detection of Human Bacteremia.

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Published in 2022 at "Small methods"

DOI: 10.1002/smtd.202101239

Abstract: The current diagnosis of bacteremia mainly relies on blood culture, which is inadequate for the rapid and quantitative determination of most bacteria in blood. Here, a quantitative, multiplex, microfluidic fluorescence in situ hybridization method (μFISH)… read more here.

Keywords: bacteremia; enables early; fluorescence situ; blood ... See more keywords
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3D Immuno-DNA Fluorescence In Situ Hybridization (FISH) for Detection of HIV-1 and Cellular Genes in Primary CD4+ T Cells.

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Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0664-3_14

Abstract: Fluorescence in situ hybridization (FISH) is a powerful, broadly used microscopy-based technique that leverages fluorescently labeled nucleic acid probes to detect parts of the genome inside metaphase or interphase cell nuclei. In recent years, different… read more here.

Keywords: microscopy; hybridization fish; fluorescence situ; immuno dna ... See more keywords
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An Introduction to Fluorescence in situ Hybridization in Microorganisms.

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Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1115-9_1

Abstract: Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of microorganisms in a sample. This technique has found applications in several areas, most notably the environmental, for… read more here.

Keywords: fluorescence situ; hybridization; introduction fluorescence; biology ... See more keywords
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Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.

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Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1115-9_3

Abstract: Fluorescence in situ hybridization (FISH) is a well-established technique that allows the detection of microorganisms in diverse types of samples (e.g., clinical, food, environmental samples, and biofilm communities). The FISH probe design is an essential… read more here.

Keywords: fluorescence situ; bioinformatic tools; situ hybridization; guidelines design ... See more keywords
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Characterization of Social Interactions and Spatial Arrangement of Individual Bacteria in MultiStrain or Multispecies Biofilm Systems Using Nucleic Acid Mimics-Fluorescence In Situ Hybridization.

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Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1115-9_7

Abstract: Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within… read more here.

Keywords: social interactions; fluorescence situ; arrangement individual; multistrain multispecies ... See more keywords
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Fluorescence In Situ Hybridization in Oat.

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Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6682-0_1

Abstract: This chapter describes methods to detect gene loci or gene transcripts by fluorescence labeling. Fluorescence in situ hybridization (FISH) can be used to identify the positions of genes or BACs or the distribution of repetitive… read more here.

Keywords: fluorescence situ; hybridization; hybridization oat; gene ... See more keywords
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Applications of Fluorescence In Situ Hybridization Technology in Malignancies.

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Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6703-2_8

Abstract: The molecular characterization of nonrandom recurrent cytogenetic abnormalities has identified numerous disease-related genes involved in hematologic and lymphoid malignancies. Cytogenetic analysis has become essential for disease diagnosis, classification, prognostic stratification, and treatment guidance. Fluorescence in… read more here.

Keywords: applications fluorescence; fluorescence situ; analysis; hybridization technology ... See more keywords