A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6958-6_3
Abstract: Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can… read more here.
Keywords: fluorescent assay; cell based; protein synthesis; based fluorescent ... See more keywords
Fluorescent Assay of FEN1 Activity with Nicking Enzyme-Assisted Signal Amplification Based on ZIF-8 for Imaging in Living Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c05473
Abstract: Flap endonuclease 1 (FEN1) participates in both DNA replication and repair to maintain the stability and integrity of the genome. As a potential tumor marker, detecting FEN1 activity could be an effective strategy for cancer… read more here.
Keywords: assay fen1; fen1 activity; nicking enzyme; living cells ... See more keywords
A novel fluorescent assay for uracil DNA glycosylase activity built on the 3′–5′ exonuclease activity-based endonuclease IV cyclic signal amplification strategy
Sign Up to like & getrecommendations! Published in 2020 at "New Journal of Chemistry"
DOI: 10.1039/d0nj04729e
Abstract: The unique 3′-5′ exonuclease activity of endonuclease IV to DNA strands has been demonstrated, which enables the development of a novel highly sensitive assay for UDG activity. read more here.
Keywords: endonuclease; novel fluorescent; activity; fluorescent assay ... See more keywords