Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage
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DOI: 10.1371/journal.pbio.3001527
Abstract: CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation of DNA double-strand breaks (DSBs) to stimulate homology-directed… read more here.
Keywords: gene tagging; fluorescent gene; human organoids; double strand ... See more keywords