UltraPlex Hapten-Based Multiplexed Fluorescent Immunohistochemistry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1593-5_17
Abstract: The UltraPlex method for multiplexed two-dimensional fluorescent immunohistochemistry is described, in which hapten tags conjugated to primary antibodies facilitate multiplexed imaging of four or more antigens per tissue section at once. Anti-hapten secondary antibodies labeled… read more here.
Keywords: ultraplex hapten; immunohistochemistry; tissue samples; hapten based ... See more keywords
Fluorescent Immunohistochemistry.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1948-3_9
Abstract: Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal… read more here.
Keywords: immunofluorescence; biology; fluorescent immunohistochemistry; fluorescence ... See more keywords
Cyclic multiplex fluorescent immunohistochemistry and machine learning reveal distinct states of astrocytes and microglia in normal aging and Alzheimer’s disease
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Neuroinflammation"
DOI: 10.1186/s12974-022-02383-4
Abstract: Background Astrocytes and microglia react to Aβ plaques, neurofibrillary tangles, and neurodegeneration in the Alzheimer’s disease (AD) brain. Single-nuclei and single-cell RNA-seq have revealed multiple states or subpopulations of these glial cells but lack spatial… read more here.
Keywords: fluorescent immunohistochemistry; machine learning; multiplex fluorescent; astrocytes microglia ... See more keywords