On‐capillary fluorescent labeling of saccharides for capillary electrophoresis
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200136
Abstract: The feasibility of on‐capillary derivatization of saccharides by aromatic amine‐based fluorescent labeling agents was tested. To avoid the problematic evolution of gaseous hydrogen cyanide, the Schiff base reduction by sodium cyanoborohydride, as the second step… read more here.
Keywords: fluorescent labeling; labeling agent; saccharides capillary; electrophoresis capillary ... See more keywords
Fluorescent Labeling of the Nuclear Envelope Without Relying on Inner Nuclear Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_1
Abstract: The nuclear envelope (NE), a double membrane that separates nuclear components from the cytoplasm, undergoes a breakdown and reformation during cell division. To trace NE dynamics, the NE needs to be labeled with a fluorescent… read more here.
Keywords: nuclear envelope; nuclear membrane; inner nuclear; fluorescent labeling ... See more keywords
A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9121-1_4
Abstract: Fluorescent labeling of de novo synthesized proteins is in particular a valuable tool for functional and structural studies of membrane proteins. In this context, we present two methods for the site-specific fluorescent labeling of difficult-to-express… read more here.
Keywords: protein; cell free; protein synthesis; fluorescent labeling ... See more keywords
A novel fluorescent labeling reagent, 2-(9-acridone)-ethyl chloroformate, and its application to the analysis of free amino acids in honey samples by HPLC with fluorescence detection and identification with online ESI-MS
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02969-y
Abstract: In this study, a novel fluorescent labeling reagent 2-(9-acridone)-ethyl chloroformate (AEC-Cl) was designed, synthesized and applied for the determination of free amino acids by high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). The free amino… read more here.
Keywords: amino; amino acids; novel fluorescent; fluorescence ... See more keywords
Direct Visualization of Individual mRNA Export through Differential Fluorescent Labeling
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1172
Abstract: The nuclear export of mRNA through the nuclear pore complex is a crucial step in gene expression and is tightly regulated through signal-recognizing proteins. Though the mRNAs in the cytoplasm are well imaged in fixed… read more here.
Keywords: export; mrna export; direct visualization; fluorescent labeling ... See more keywords
Fluorescent Labeling of Human Serum Albumin by Thiol-Cyanimide Addition and Its Application in the Fluorescence Quenching Method for Nanoparticle-Protein Interactions.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04231
Abstract: A boron-dipyrromethene (BODIPY)-based fluorescent probe, BDP-CN, was synthesized in this work. It had a fluorescence emission maximum at 512 nm and a high quantum yield (48%). As evidenced by agarose gel electrophoresis and liquid chromatography-mass… read more here.
Keywords: fluorescent labeling; addition; fluorescence quenching; labeling human ... See more keywords
Design and Applications of a Fluorescent Labeling Technique for Lipid and Surfactant Preformed Vesicles
Sign Up to like & getrecommendations! Published in 2019 at "ACS Omega"
DOI: 10.1021/acsomega.9b01094
Abstract: Amphiphilic molecules such as surfactants, lipids, and block copolymers can be assembled into bilayers and form vesicles. Fluorescent membrane labeling methods require the use of dye molecules that can be inserted into the bilayers at… read more here.
Keywords: design applications; labeling technique; applications fluorescent; preformed vesicles ... See more keywords
Facile Method for Fluorescent Labeling of Starch Nanocrystal
Sign Up to like & getrecommendations! Published in 2017 at "ACS Sustainable Chemistry & Engineering"
DOI: 10.1021/acssuschemeng.6b02157
Abstract: Fluorescently labeled starch nanocrystal (FL-SNC) was synthesized using a simple, low-cost, and scalable two-step chemical modification process. Reactive amino groups were introduced onto the SNC surface through silanization with 3-aminopropyl triethoxysilane (APTES), and fluorescein isothiocyanate… read more here.
Keywords: facile method; method fluorescent; spectroscopy; fluorescent labeling ... See more keywords
An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc00850c
Abstract: The iminodiacetate-Ni2+-hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization… read more here.
Keywords: polyelectrolyte fluorescent; modified conjugated; iminodiacetate modified; fluorescent labeling ... See more keywords
Diyne Inactivators and Activity-Based Fluorescent Labeling of Phenol Hydroxylase in Pseudomonas sp. CF600.
Sign Up to like & getrecommendations! Published in 2023 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnad002
Abstract: An activity-based labeling (ABL) approach was investigated for the phenol-oxidizing bacterium, Pseudomonas sp. CF600. Phenol-grown cells were exposed to several different terminal diynes and following cell breakage, extracts of these cells were added to copper-catalyzed… read more here.
Keywords: pseudomonas cf600; fluorescent labeling; activity; phenol hydroxylase ... See more keywords
Fluorescent labeling of endogenous platelets for intravital microscopy: Effects on platelet function
Sign Up to like & getrecommendations! Published in 2018 at "Microcirculation"
DOI: 10.1111/micc.12457
Abstract: Monitoring endogenous platelets during intravital microscopy often involves two approaches: fluorescently labeled antibodies or genetic models of platelet‐specific fluorescent protein expression. Due to limited data available on platelet functional changes induced by these methods, we… read more here.
Keywords: microscopy; intravital microscopy; endogenous platelets; fluorescent labeling ... See more keywords