Phenylalanine stacking enhances the red fluorescence of biliverdin IXα on UV excitation in sandercyanin fluorescent protein
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14281
Abstract: Biliverdin IXα (BV) binds to several prokaryotic and eukaryotic proteins. How nature exploits the versatility of BV's properties is not fully understood. Unlike free BV, the Sandercyanin fluorescent protein bound to BV (SFP‐BV) shows enhanced… read more here.
Keywords: fluorescent protein; sandercyanin fluorescent; biliverdin; red fluorescence ... See more keywords
Fracture Detection in Bio‐Glues with Fluorescent‐Protein‐Based Optical Force Probes
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Materials"
DOI: 10.1002/adma.202210052
Abstract: Glues are being used to bond, seal, and repair in industry and biomedicine. The improvement of gluing performance is hence important for the development of new glues with better and balanced property spaces, which in… read more here.
Keywords: optical force; fluorescent protein; bio glues; force probes ... See more keywords
A Large Stokes Shift Fluorescent Protein Constructed from the Fusion of Red Fluorescent mCherry and Far‐Red Fluorescent BDFP1.6
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800695
Abstract: Phycobiliproteins are constituents of phycobilisomes that can harvest orange, red, and far‐red light for photosynthesis in cyanobacteria and red algae. Phycobiliproteins in the phycobilisome cores, such as allophycocyanins, absorb far‐red light to funnel energy to… read more here.
Keywords: large stokes; red fluorescent; fluorescent protein; mcherry ... See more keywords
Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_9
Abstract: Advances in live-cell imaging have been accelerated by the development of various fluorescent indicators. However, indicators that are suitable for multicolor imaging remain a challenge to develop. Herein, we have developed a single fluorescent protein… read more here.
Keywords: single fluorescent; protein based; indicator; fluorescent protein ... See more keywords
Green Fluorescent Protein Expression in Pseudogymnoascus destructans to Study Its Abiotic and Biotic Lifestyles
Sign Up to like & getrecommendations! Published in 2018 at "Mycopathologia"
DOI: 10.1007/s11046-018-0285-2
Abstract: Pseudogymnoascus destructans (Pd) is the etiologic agent of bat White-nose syndrome, a disease that has caused the unprecedented reduction in the hibernating bat populations across eastern North America. The Pd pathogenesis appears to be a… read more here.
Keywords: green fluorescent; fluorescent protein; expression; biology ... See more keywords
Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles
Sign Up to like & getrecommendations! Published in 2019 at "Russian Chemical Bulletin"
DOI: 10.1007/s11172-019-2524-1
Abstract: The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional… read more here.
Keywords: immobilization; fluorescent protein; taggfp2 fe3o4; protein taggfp2 ... See more keywords
SKLPT imaging: Efficient in vivo pre-evaluation of genome-editing modules using fluorescent protein with peroxisome targeting signal.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.06.008
Abstract: Numerous studies have used genome-editing modules such as CRISPR-Cas9 for site-directed mutagenesis; however, evaluation of the efficiency of these modules remains a time-consuming process. Here, we report the development of SKL-mediated Peroxisome Targeting Imaging (SKLPT… read more here.
Keywords: genome editing; sklpt imaging; pre; fluorescent protein ... See more keywords
Red emission cysteine probe with high selectivity based on fluorescent protein chromophores and turn-on fluorescence in cell cultures
Sign Up to like & getrecommendations! Published in 2019 at "Dyes and Pigments"
DOI: 10.1016/j.dyepig.2019.03.034
Abstract: Abstract Cysteine (Cys) plays a vital role in many physiological processes, and also is closely related to various diseases, thus the development of novel high selectivity and sensitivity fluorescent probes for in situ monitoring of… read more here.
Keywords: protein chromophores; cys; based fluorescent; probe ... See more keywords
Green fluorescent protein chromophore derivatives as a new class of aldose reductase inhibitors.
Sign Up to like & getrecommendations! Published in 2017 at "European journal of medicinal chemistry"
DOI: 10.1016/j.ejmech.2016.10.016
Abstract: A number of (Z)-4-arylmethylene-1H-imidazol-5(4H)-ones, which are related to the fluorescent chromophore of the Aequorea green fluorescent protein (GFP), have been synthesized and evaluated their in vitro inhibitory activity against recombinant human aldose reductase for the first… read more here.
Keywords: aldose reductase; green fluorescent; chromophore; fluorescent protein ... See more keywords
Analysis and modeling of enhanced green fluorescent protein diffusivity in whey protein gels.
Sign Up to like & getrecommendations! Published in 2019 at "Food research international"
DOI: 10.1016/j.foodres.2018.10.087
Abstract: During gastric digestion, hydrolysis of proteins by pepsin contributes largely to the breakdown of protein-rich food. We hypothesized that the effect of pepsin is limited by its diffusivity, which is co-determined by the food structure… read more here.
Keywords: diffusivity; enhanced green; food; green fluorescent ... See more keywords
Probing interdomain linkers and protein supertertiary structure in vitro and in live cells with fluorescent protein resonance energy transfer.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2020.166793
Abstract: Many proteins are composed of independently-folded domains connected by flexible linkers. The primary sequence and length of such linkers can set the effective concentration for the tethered domains, which impacts rates of association and enzyme… read more here.
Keywords: interdomain linkers; length; energy transfer; fluorescent protein ... See more keywords