It's a two‐way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14609
Abstract: Chromophore‐bearing proteins that are (reversibly) altered after light illumination are major functional components of nature. They gained considerable attention in the last decades since the dynamic interactions of the chromophore and protein matrix can be… read more here.
Keywords: fluorescent proteins; two way; way street; protein matrix ... See more keywords
Reactor control system in bacterial co-culture based on fluorescent proteins using an Arduino-based home-made device.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology journal"
DOI: 10.1002/biot.202100169
Abstract: BACKGROUND Co-culture, fermentation with more than two microbial strains, is a potential flexible method for optimizing the metabolic conversion process in bio-production. However, maintaining an ideal population throughout the fermentation process remains a challenge. METHODS… read more here.
Keywords: fluorescent proteins; culture; population; control system ... See more keywords
Building a spectral cytometry toolbox: Coupling fluorescent proteins and antibodies to microspheres
Sign Up to like & getrecommendations! Published in 2022 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24557
Abstract: Fluorescent proteins (FPs) have become an essential tool for biological research. Since the isolation and description of GFP, hundreds of fluorescent proteins have been discovered and created with various characteristics. The excitation of these proteins… read more here.
Keywords: fluorescent proteins; cytometry toolbox; building spectral; toolbox coupling ... See more keywords
From fluorescent proteins to fluorogenic RNAs: Tools for imaging cellular macromolecules
Sign Up to like & getrecommendations! Published in 2019 at "Protein Science"
DOI: 10.1002/pro.3632
Abstract: The explosion in genome‐wide sequencing has revealed that noncoding RNAs are ubiquitous and highly conserved in biology. New molecular tools are needed for their study in live cells. Fluorescent RNA–small molecule complexes have emerged as… read more here.
Keywords: cellular macromolecules; fluorogenic rnas; fluorescent proteins; proteins fluorogenic ... See more keywords
To twist or not to twist: From chromophore structure to dynamics inside engineered photoconvertible and photoswitchable fluorescent proteins
Sign Up to like & getrecommendations! Published in 2022 at "Protein Science"
DOI: 10.1002/pro.4517
Abstract: Green‐to‐red photoconvertible fluorescent proteins (FPs) are vital biomimetic tools for powerful techniques such as super‐resolution imaging. A unique Kaede‐type FP named the least evolved ancestor (LEA) enables delineation of the evolutionary step to acquire photoconversion… read more here.
Keywords: fluorescent proteins; chromophore structure; lea; structure dynamics ... See more keywords
Kinetic isotope effect reveals rate‐limiting step in green‐to‐red photoconvertible fluorescent proteins
Sign Up to like & getrecommendations! Published in 2024 at "Protein Science"
DOI: 10.1002/pro.5069
Abstract: Photoconvertible fluorescent proteins (pcFPs) undergo a slow photochemical transformation when irradiated with blue light. Since their emission is shifted from green to red, pcFPs serve as convenient fusion tags in several cutting‐edge biological imaging technologies.… read more here.
Keywords: fluorescent proteins; photoconvertible fluorescent; rate limiting; rate ... See more keywords
Structural insights into the fusion of annexin A5 and fluorescent proteins generating hundredfold differentiated binding affinities to phosphatidylserine
Sign Up to like & getrecommendations! Published in 2025 at "Protein Science"
DOI: 10.1002/pro.70086
Abstract: Fluorescent proteins (FPs) are an indispensable part of modern biology. Numerous studies utilize FPs for protein labeling and cell tracking purposes. They are commonly fused with proteins to aid in their visualization. It is generally… read more here.
Keywords: fluorescent proteins; fusion; fps; anxa5 probes ... See more keywords
Bacterial Chemoreceptor Imaging at High Spatiotemporal Resolution Using Photoconvertible Fluorescent Proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7577-8_18
Abstract: We describe two methods for high-resolution fluorescence imaging of the positioning and mobility of E. coli chemoreceptors fused to photoconvertible fluorescent proteins. Chemoreceptors such as Tar and Tsr are transmembrane proteins expressed at high levels… read more here.
Keywords: resolution; photoconvertible fluorescent; bacterial chemoreceptor; fluorescent proteins ... See more keywords
Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9593-6_4
Abstract: Vaccinia virus recombinants that express fluorescent proteins have a variety of applications such as the identification of infected cells, efficient screening for genetically modified strains, and molecular characterization of virus replication and spread. The detection… read more here.
Keywords: construction; fluorescent proteins; vaccinia virus; isolation ... See more keywords
A short guide on blue fluorescent proteins: limits and perspectives
Sign Up to like & getrecommendations! Published in 2024 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-024-13012-w
Abstract: Abstract The advent of the so-called colorful biology era is in line with the discovery of fluorescent proteins (FPs), which can be widely used to detect the intracellular locations of macromolecules or to determine the… read more here.
Keywords: fluorescent proteins; blue fluorescent; fluorescent; nadph dependent ... See more keywords
Spectroscopic Analysis of the Cu2+-Induced Fluorescence Quenching of Fluorescent Proteins AmCyan and mOrange2
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biotechnology"
DOI: 10.1007/s12033-018-0088-1
Abstract: Fluorescent proteins show fluorescence quenching by specific metal ions, which can be applied towards metal biosensing applications. In order to develop metal-biosensor, we performed spectroscopic analysis of the fluorescence quenching of fluorescent protein AmCyan and… read more here.
Keywords: fluorescence quenching; amcyan morange2; fluorescence; fluorescent proteins ... See more keywords