Increased expression of the fluorescent reporter protein ymNeonGreen in Saccharomyces cerevisiae by reducing RNA secondary structure near the start codon.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Reports"
DOI: 10.1016/j.btre.2021.e00697
Abstract: Highlights • Stable secondary RNA structure 3′ of the start codon inhibits ymNeonGreen expression.• Removing secondary RNA structure increased expression in S. cerevisiae and E. coli.• Expression was increased 2-fold in S. cerevisiae.• Expression was… read more here.
Keywords: fluorescent reporter; expression; start codon; increased expression ... See more keywords
Establishment of Green- and Red-Fluorescent Reporter Proteins Based on the Fluorescence-Activating and Absorption-Shifting Tag for Use in Acetogenic and Solventogenic Anaerobes.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00554
Abstract: Anaerobic bacteria are promising biocatalysts to produce industrially relevant products from nonfood feedstocks. Several anaerobes are genetically accessible, and various molecular tools for metabolic engineering are available. Still, the use of bright fluorescent reporters, which… read more here.
Keywords: establishment green; fluorescent reporter; fluorescent; reporter proteins ... See more keywords
Screening for inhibitors of mutacin synthesis in Streptococcus mutans using fluorescent reporter strains
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DOI: 10.1186/s12866-018-1170-3
Abstract: BackgroundWithin the polymicrobial dental plaque biofilm, bacteria kill competitors by excreting mixtures of bacteriocins, resulting in improved fitness and survival. Inhibiting their bacteriocin synthesis might therefore be a useful strategy to eliminate specific pathogens. We… read more here.
Keywords: reporter; mutacin synthesis; fluorescent reporter; synthesis ... See more keywords
A fluorescent reporter on electrostatic DNA-ligand interactions.
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DOI: 10.1364/boe.439791
Abstract: Among the various types of interactions between biomolecules, electrostatic interactions dominate as these are long-range interactions and are often a generic first step in the recruitment of specific ligands. DNA, being a highly charged molecule,… read more here.
Keywords: dna; fluorescent reporter; ligand interactions; dna ligand ... See more keywords
PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells
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DOI: 10.7554/elife.69504
Abstract: Prime editing is a recently developed CRISPR/Cas9 based gene engineering tool that allows the introduction of short insertions, deletions, and substitutions into the genome. However, the efficiency of prime editing, which typically achieves editing rates… read more here.
Keywords: enrichment; fluorescent reporter; reporter; prime editing ... See more keywords