In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent In Situ Hybridization (FISH).
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0621-6_23
Abstract: The arabinogalactan proteins are highly glycosylated and ubiquitous in plants. They are involved in several aspects of plant development and reproduction; however, the mechanics behind their function remains for the most part unclear, as the… read more here.
Keywords: protein; fluorescent situ; hybridization fish; situ ... See more keywords
Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_15
Abstract: In situ transcriptomic methods hold immense promise for spatially resolved mapping of cell types across human tissues. Here, we describe a protocol for automated single-molecule fluorescent in situ hybridization (smFISH) on standard histology sections at… read more here.
Keywords: fluorescent situ; single molecule; molecule fluorescent; rnascope ... See more keywords
Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_3
Abstract: Fluorescent in situ hybridization (FISH) techniques can be used to identify a range of chromosome abnormalities that are clinically significant in many cancers. Multicolor FISH can be used to identify multiple targets, which can be… read more here.
Keywords: fluorescent situ; practical application; application fluorescent; situ hybridization ... See more keywords
Higher-Order Chromatin Organization Using 3D DNA Fluorescent In Situ Hybridization.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0664-3_13
Abstract: A comprehensive analysis of the tridimensional (3D) organization of the genome is crucial to understand gene regulation. Three-dimensional DNA fluorescent in situ hybridization (3D-FISH) is a method of choice to study nuclear organization at the… read more here.
Keywords: fluorescent situ; microscopy; chromatin; dna fluorescent ... See more keywords
Following Trypanosoma cruzi RPA-DNA Interaction Using Fluorescent In Situ Hybridization Coupled with Immunofluorescence (FISH/IF).
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_12
Abstract: Fluorescent in situ hybridization coupled with immunofluorescence (FISH/IF) is an assay that has been widely used to study DNA-protein interactions. The technique is based on the use of a fluorescent nucleic acid probe and fluorescent… read more here.
Keywords: fluorescent situ; dna; hybridization coupled; interaction ... See more keywords
Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7144-2_10
Abstract: Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs… read more here.
Keywords: fluorescent situ; hybridization fish; tumor cells; circulating tumor ... See more keywords
Sex determination from fingermarks using fluorescent in situ hybridization
Sign Up to like & getrecommendations! Published in 2018 at "Analytical Methods"
DOI: 10.1039/c7ay02805a
Abstract: When fingermarks are not suited for automated fingerprint identification, caused by for instance, poor development or when no match can be found in the fingerprint database, the chemical composition can be used to extract additional… read more here.
Keywords: fluorescent situ; dna; donor; sex ... See more keywords
dotdotdot: an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa312
Abstract: Abstract Multiplex single-molecule fluorescent in situ hybridization (smFISH) is a powerful method for validating RNA sequencing and emerging spatial transcriptomic data, but quantification remains a computational challenge. We present a framework for generating and analyzing… read more here.
Keywords: fluorescent situ; single molecule; multiplex single; complex tissues ... See more keywords
Use of fluorescent in‐situ hybridisation in salivary gland cytology: A powerful diagnostic tool
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DOI: 10.1111/cyt.12427
Abstract: Salivary gland cytology is challenging because it includes a diversity of lesions and a wide spectra of tumours. Recently, it has been reported that many types of salivary gland tumours have specific molecular diagnostic signatures… read more here.
Keywords: fluorescent situ; situ hybridisation; salivary gland; gland cytology ... See more keywords
Different Translocations of Multiple Myeloma on Fluorescent In Situ Hybridization (FISH) with Clinical Correlation.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the College of Physicians and Surgeons--Pakistan : JCPSP"
DOI: 10.29271/jcpsp.2023.03.281
Abstract: OBJECTIVE To evaluate the association of chromosomal translocations in multiple myeloma (MM) detected by Fluorescent In Situ Hybridization (FISH) and its clinical characteristics. STUDY DESIGN Cross-sectional study. Place and Duration of the Study: Department of… read more here.
Keywords: situ hybridization; translocations multiple; hybridization fish; fluorescent situ ... See more keywords
Rapid Characterization of Microcystin-Producing Cyanobacteria in Freshwater Lakes by TSA-FISH (Tyramid Signal Amplification-Fluorescent In Situ Hybridization)
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Environmental Science"
DOI: 10.3389/fenvs.2017.00043
Abstract: Microcystin (MC) is a common and widespread toxin which represents a health hazard to humans and animals. MC toxin concentrations are monitored by various direct or proxy techniques (HPLC, LC-MS/MS, ELISA, PPIA), however, these techniques… read more here.
Keywords: fluorescent situ; tyramid signal; tsa fish; signal amplification ... See more keywords