Fluorescence Lifetime Multiplexing with Fluorogen-Activating FAST Protein Variants and Red-Shifted Arylidene–Imidazolone Derivative as Fluorogen
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DOI: 10.3390/bios15050274
Abstract: Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed imaging in live cells. In this work, we present a genetically encoded FLIM multiplexing platform based on a combination of fluorogen-activating protein FAST and… read more here.
Keywords: fluorescence lifetime; fluorogen; fast protein; arylidene imidazolone ... See more keywords
Spatial Structure of NanoFAST in the Apo State and in Complex with its Fluorogen HBR-DOM2
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DOI: 10.3390/ijms231911361
Abstract: NanoFAST is a fluorogen-activating protein and can be considered one of the smallest encodable fluorescent tags. Being a shortened variant of another fluorescent tag, FAST, nanoFAST works nicely only with one out of all known… read more here.
Keywords: spatial structure; apo state; structure; fluorogen ... See more keywords