Fluorogenic RNA-Based Biosensor to Sense the Glycolytic Flux in Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00100
Abstract: The visualization of metabolic flux in real time requires sensor molecules that transduce variations of metabolite concentrations into an appropriate output signal. In this regard, fluorogenic RNA-based biosensors are promising molecular tools as they fluoresce… read more here.
Keywords: mammalian cells; fluorogenic rna; based biosensor; rna based ... See more keywords
A fluorogenic RNA aptamer nanodevice for the low background imaging of mRNA in living cells.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d1cc06582c
Abstract: A fluorogenic RNA aptamer nanodevice integrating an entropy-driven RNA amplifier with near-infrared (NIR) light control was developed, affording high contrast and sensitivity for imaging low-abundance mRNA in living cells. The design principle offers a new… read more here.
Keywords: aptamer nanodevice; rna aptamer; low background; living cells ... See more keywords
Transcriptionally amplified synthesis of fluorogenic RNA aptamers for label-free DNA glycosylase assay.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc03628b
Abstract: We demonstrate for the first time the utilization of fluorogenic RNA aptamers for label-free uracil-DNA glycosylase (UDG) assay. Through rationally engineering the transcription machine with dU substitution, this assay requires only a single probe to… read more here.
Keywords: label free; dna glycosylase; rna aptamers; aptamers label ... See more keywords