Articles with "fluorometric assays" as a keyword



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Enzymatic fluorometric assays for quantifying all major phospholipid classes in cells and intracellular organelles

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Published in 2019 at "Scientific Reports"

DOI: 10.1038/s41598-019-45185-0

Abstract: Cell membrane phospholipids regulate various biological functions. We previously reported enzymatic fluorometric methods for quantifying phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin, phosphatidylglycerol and cardiolipin. In the present report, a new enzymatic fluorometric assay was developed… read more here.

Keywords: enzymatic fluorometric; phospholipid classes; intracellular organelles; cells intracellular ... See more keywords
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Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes

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Published in 2020 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms21031032

Abstract: Phospholipids, consisting of a hydrophilic head group and two hydrophobic acyl chains, are essential for the structures of cell membranes, plasma lipoproteins, biliary mixed micelles, pulmonary surfactants, and extracellular vesicles. Beyond their structural roles, phospholipids… read more here.

Keywords: enzymatic fluorometric; phospholipid classes; assays quantify; protocols enzymatic ... See more keywords