Metabolic modelling and flux analysis of microorganisms from the Atacama Desert used in biotechnological processes
Sign Up to like & getrecommendations! Published in 2018 at "Antonie van Leeuwenhoek"
DOI: 10.1007/s10482-018-1031-y
Abstract: Metabolic modelling is a useful tool that enables the rational design of metabolic engineering experiments and the study of the unique capabilities of biotechnologically important microorganisms. The extreme abiotic conditions of the Atacama Desert have… read more here.
Keywords: flux analysis; modelling flux; atacama desert; metabolic modelling ... See more keywords
Isotopically nonstationary metabolic flux analysis (INST-MFA): putting theory into practice.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2018.02.013
Abstract: Typically, 13C flux analysis relies on assumptions of both metabolic and isotopic steady state. If metabolism is steady but isotope labeling is not allowed to fully equilibrate, isotopically nonstationary metabolic flux analysis (INST-MFA) can be… read more here.
Keywords: mfa; flux analysis; inst mfa;
Biological insights into non-model microbial hosts through stable-isotope metabolic flux analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2019.09.010
Abstract: Stable-isotope metabolic flux analysis is an important approach to unravel the metabolic network and its regulation in organisms. It has become a key analytical technology for biotechnological applications. During recent years non-model microorganisms have received… read more here.
Keywords: metabolic flux; stable isotope; flux analysis; isotope metabolic ... See more keywords
13C metabolic flux analysis of three divergent extremely thermophilic bacteria: Geobacillus sp. LC300, Thermus thermophilus HB8, and Rhodothermus marinus DSM 4252.
Sign Up to like & getrecommendations! Published in 2017 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2017.10.007
Abstract: Thermophilic organisms are being increasingly investigated and applied in metabolic engineering and biotechnology. The distinct metabolic and physiological characteristics of thermophiles, including broad substrate range and high uptake rates, coupled with recent advances in genetic… read more here.
Keywords: metabolic flux; engineering; 13c metabolic; three divergent ... See more keywords
Comprehensive Isotopic Targeted Mass Spectrometry (CIT-MS): Reliable Metabolic Flux Analysis with Broad Coverage.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c01767
Abstract: Metabolic flux analysis (MFA) is highly relevant to understanding metabolic mechanisms of various biological processes. While the pace of methodology development in MFA has been rapid, a major challenge the field continues to witness is… read more here.
Keywords: mass spectrometry; cit; analysis; flux ... See more keywords
Novel standardized method for extracellular flux analysis of oxidative and glycolytic metabolism in peripheral blood mononuclear cells
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-81217-4
Abstract: Analyzing metabolism of peripheral blood mononuclear cells (PBMCs) provides key opportunities to study the pathophysiology of several diseases, such as type 2 diabetes, obesity and cancer. Extracellular flux (XF) assays provide dynamic metabolic analysis of… read more here.
Keywords: pbmcs; flux analysis; analysis; method ... See more keywords
Mass, Energy, and Momentum Flux Analysis of a Bendway Weir
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Hydraulic Engineering"
DOI: 10.1061/(asce)hy.1943-7900.0001349
Abstract: AbstractBendway weirs are hydraulic control structures found in watercourses that range in scale from small streams to large rivers. They are designed to be submerged, broad-crested weirs, typicall... read more here.
Keywords: energy momentum; momentum flux; mass energy; analysis bendway ... See more keywords
Reversible jump MCMC for multi-model inference in Metabolic Flux Analysis
Sign Up to like & getrecommendations! Published in 2020 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btz500
Abstract: MOTIVATION The validity of model based inference, as used in systems biology, depends on the underlying model formulation. Often, a vast number of competing models is available, that are built on different assumptions, all consistent… read more here.
Keywords: metabolic flux; multi model; model; flux analysis ... See more keywords
Extracellular flux analysis in intact cardiac tissue slices - a novel tool to investigate cardiac substrate metabolism in mouse myocardium.
Sign Up to like & getrecommendations! Published in 2023 at "Acta physiologica"
DOI: 10.1111/apha.14004
Abstract: AIM Cardiac pathologies are accompanied by alterations in substrate metabolism, and extracellular flux analysis is a standard tool to investigate metabolic disturbances, especially in immortalized cell lines. However, preparations of primary cells, such as adult… read more here.
Keywords: metabolism; tissue slices; extracellular flux; flux analysis ... See more keywords
Abstract 1160: A machine learning based method for in-vivo metabolic flux analysis of patient tumors
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-1160
Abstract: Metabolic fluxes likely control cancer phenotypes and treatment responses but cannot be measured in cancers in human patients. Machine learning has become a popular tool to study cancer biology and can identify complex patterns in… read more here.
Keywords: machine; machine learning; flux analysis; metabolic flux ... See more keywords
Metabolic flux analysis for metabolome data validation of naturally xylose-fermenting yeasts
Sign Up to like & getrecommendations! Published in 2019 at "BMC Biotechnology"
DOI: 10.1186/s12896-019-0548-0
Abstract: BackgroundEfficient xylose fermentation still demands knowledge regarding xylose catabolism. In this study, metabolic flux analysis (MFA) and metabolomics were used to improve our understanding of xylose metabolism. Thus, a stoichiometric model was constructed to simulate… read more here.
Keywords: metabolic flux; metabolome data; flux analysis; data validation ... See more keywords