Optimizing performance of semi‐continuous cell culture in an ambr15™ microbioreactor using dynamic flux balance modeling
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2585
Abstract: The ambr bioreactors are single‐use microbioreactors for cell line development and process optimization. With operating conditions for large‐scale biopharmaceutical production properly scaled down, microbioreactors such as the ambr15™ can potentially be used to predict the… read more here.
Keywords: flux balance; production; process; dynamic flux ... See more keywords
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3313
Abstract: Therapeutic protein productivity and glycosylation pattern highly rely on cell metabolism. Cell culture medium composition and feeding strategy are critical to regulate cell metabolism. In this study, the relationship between toxic metabolic inhibitors and their… read more here.
Keywords: cell; cell metabolism; flux balance; nutrient precursors ... See more keywords
Competitive, multi-objective and compartmented Flux Balance Analysis for addressing tissue specific Inborn Errors of Metabolism.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of inherited metabolic disease"
DOI: 10.1002/jimd.12603
Abstract: The inborn error of metabolism Phenylketonuria (PKU, OMIM 261600) is most often due to inactivation of phenylalanine hydroxylase (PAH), which converts phenylalanine (Phe) into tyrosine (Tyr). The reduced PAH activity increases blood concentration of phenylalanine… read more here.
Keywords: flux balance; brain; pathology; blood ... See more keywords
A systematic approach for finding the objective function and active constraints for dynamic flux balance analysis
Sign Up to like & getrecommendations! Published in 2018 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-018-1899-y
Abstract: Dynamic flux balance analysis (DFBA) has become an instrumental modeling tool for describing the dynamic behavior of bioprocesses. DFBA involves the maximization of a biologically meaningful objective subject to kinetic constraints on the rate of… read more here.
Keywords: flux balance; objective function; balance analysis; approach finding ... See more keywords
Understanding regulation in substrate dependent modulation of growth and production of alcohols in Clostridium sporogenes NCIM 2918 through metabolic network reconstruction and flux balance analysis.
Sign Up to like & getrecommendations! Published in 2018 at "Bioresource technology"
DOI: 10.1016/j.biortech.2017.10.080
Abstract: Flux Balance Analysis was performed for Clostridium sporogenes NCIM 2918 grown on sole glucose and glycerol or glucose-glycerol combinations at varied concentrations. During acidogenesis, glucose and glucose-glycerol combinations favored improved growth and butyric acid production.… read more here.
Keywords: flux balance; balance analysis; glycerol; clostridium sporogenes ... See more keywords
Interplay of Shadow Price, Phenotype and Alternative Optimal Solutions in Flux Balance Analysis
Sign Up to like & getrecommendations! Published in 2019 at "IFAC-PapersOnLine"
DOI: 10.1016/j.ifacol.2019.06.040
Abstract: Abstract Genome-scale metabolic network models (GEMs) have been applied successfully in many applications, and one important application is to predict different growth phenotypes in various genetic and environmental conditions. Phenotype phase plane (PhPP) analysis is… read more here.
Keywords: shadow price; phpp analysis; flux balance; analysis ... See more keywords
Flux Balance Analysis for Media Optimization and Genetic Targets to Improve Heterologous Siderophore Production
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DOI: 10.1016/j.isci.2020.101016
Abstract: Summary Siderophores are small molecule metal chelators secreted in sparse quantities by their native microbial hosts but can be engineered for enhanced production from heterologous hosts like Escherichia coli. These molecules have been proved to… read more here.
Keywords: flux balance; production; heterologous siderophore; improve heterologous ... See more keywords
Regulatory dynamic enzyme-cost flux balance analysis: A unifying framework for constraint-based modeling.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of theoretical biology"
DOI: 10.1016/j.jtbi.2020.110317
Abstract: Integrated modeling of metabolism and gene regulation continues to be a major challenge in computational biology. While there exist approaches like regulatory flux balance analysis (rFBA), dynamic flux balance analysis (dFBA), resource balance analysis (RBA)… read more here.
Keywords: flux balance; balance analysis; biology; dynamic enzyme ... See more keywords
Comment on ‘Open-boundary spectral and flux-balance Vlasov simulation by A. Klimas and A. Viñas’
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Plasma Physics"
DOI: 10.1017/s0022377820000616
Abstract: An error in Klimas & Viñas (J. Plasma Phys., vol. 85 (6), 2019, 905850610) is noted and explained. It is shown that the results in Klimas and Viñas were unaffected by the error. Further ramifications… read more here.
Keywords: open boundary; comment open; spectral flux; balance vlasov ... See more keywords
FlySilico: Flux balance modeling of Drosophila larval growth and resource allocation
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-53532-4
Abstract: Organisms depend on a highly connected and regulated network of biochemical reactions fueling life sustaining and growth promoting functions. While details of this metabolic network are well established, knowledge of the superordinate regulatory design principles… read more here.
Keywords: resource allocation; growth; flux balance; flysilico flux ... See more keywords
Bridging substrate intake kinetics and bacterial growth phenotypes with flux balance analysis incorporating proteome allocation
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-61174-0
Abstract: Empirical kinetic models such as the Monod equation have been widely applied to relate the cell growth with substrate availability. The Monod equation shares a similar form with the mechanistically-based Michaelis-Menten kinetics for enzymatic processes,… read more here.
Keywords: flux balance; growth phenotypes; balance analysis; proteome allocation ... See more keywords