Focused classification and refinement in high-resolution cryo-EM structural analysis of ribosome complexes.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2017.07.007
Abstract: Cryo electron microscopy (cryo-EM) historically has had a strong impact on the structural and mechanistic analysis of protein synthesis by the prokaryotic and eukaryotic ribosomes. Vice versa, studying ribosomes has helped moving forwards many methodological… read more here.
Keywords: high resolution; analysis; focused classification; biology ... See more keywords
Analysis of discrete local variability and structural covariance in macromolecular assemblies using Cryo-EM and focused classification.
Sign Up to like & getrecommendations! Published in 2019 at "Ultramicroscopy"
DOI: 10.1016/j.ultramic.2018.11.016
Abstract: Single-particle electron cryo-microscopy and computational image classification can be used to analyze structural variability in macromolecules and their assemblies. In some cases, a particle may contain different regions that each display a range of distinct… read more here.
Keywords: covariance; variability; focused classification; cryo ... See more keywords