One-Shot In Vitro Evolution Generated an Antibody Fragment for Testing Urinary Cotinine with More Than 40-Fold Enhanced Affinity.
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DOI: 10.1021/acs.analchem.6b04332
Abstract: Immunoassays for cotinine, a major nicotine metabolite, in the urine are useful for monitoring the degree of tobacco smoke exposure. However, hybridoma-based anti-cotinine antibodies lack sufficient binding affinity to perform practically sensitive measurements, and thus… read more here.
Keywords: urinary cotinine; vitro evolution; one shot; cotinine ... See more keywords