Direct Measurements of the Cobalt‐Thiolate Bonds Strength in Rubredoxin by Single‐Molecule Force Spectroscopy
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200165
Abstract: Cobalt is a trace transition metal. Although it is not abundant on earth, tens of cobalt‐containing proteins exist in life. Moreover, the characteristic spectrum of Co(II) ion makes it a powerful probe for the characterization… read more here.
Keywords: single molecule; spectroscopy; force spectroscopy; force ... See more keywords
Microfluidics-Based Force Spectroscopy Enables High-Throughput Force Experiments with Sub-Nanometer Resolution and Sub-Piconewton Sensitivity.
Sign Up to like & getrecommendations! Published in 2023 at "Small"
DOI: 10.1002/smll.202206713
Abstract: Several techniques have been established to quantify the mechanicals of single molecules. However, most of them show only limited capabilities of parallelizing the measurement by performing many individual measurements simultaneously. Herein, a microfluidics-based single-molecule force… read more here.
Keywords: microfluidics based; resolution sub; spectroscopy; sub nanometer ... See more keywords
Complex Dynamics in Single Molecule Force Spectroscopy from Simple Simulation Models
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1085
Abstract: Single molecule force spectroscopy studies of protein folding dynamics have yielded unprecedented insight into the mechanisms of the folding reaction. Still, they usually allow for a very limited description of the process of interest (e.g.… read more here.
Keywords: single molecule; force spectroscopy; force; spectroscopy ... See more keywords
Bimodal Force Spectroscopy as a Technique to Determine the Young's Moduli of Protein Fibrils and Nanoparticles
Sign Up to like & getrecommendations! Published in 2017 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927617006316
Abstract: Colloidal protein structures have many applications in foods and packaging, some of which are dependent upon the mechanical attributes of the colloidal structures. Deformability of colloidal materials defines their capacity to operate as emulsifiers [1]… read more here.
Keywords: force spectroscopy; bimodal force; spectroscopy technique; determine young ... See more keywords
Ensemble Force Spectroscopy of a G-Quadruplex Cluster on a Single-Molecule Platform.
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.2c00959
Abstract: Single-molecule methods offer high sensitivities with precisions superior to bulk assays. However, these methods are low in throughput and cannot repetitively interrogate the same cluster of molecular units. In this work, we investigate a tandem… read more here.
Keywords: single molecule; spectroscopy; cluster; force spectroscopy ... See more keywords
Single-Molecule Force Spectroscopy Reveals Adhesion-by-Demand in Statherin at the Protein–Hydroxyapatite Interface
Sign Up to like & getrecommendations! Published in 2020 at "Langmuir"
DOI: 10.1021/acs.langmuir.0c02325
Abstract: Achieving strong adhesion in wet environments remains a technological challenge in biomedical applications demanding biocompatibility. Attention for adhesive motifs meeting such demands has largely been focused on marine organisms. However, bioadhesion to inorganic surfaces is… read more here.
Keywords: single molecule; force spectroscopy; adhesion; spectroscopy ... See more keywords
Force Spectroscopy on a Cell Drum: AFM Measurements on the Basolateral Side of Cells via Inverted Cell Cultures.
Sign Up to like & getrecommendations! Published in 2018 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b01990
Abstract: The elasticity of a cell is one of the most critical measures of the difference between cancerous cells and healthy cells: cancer cells tend to be softer than healthy cells, and highly invasive cells tend… read more here.
Keywords: drum; force spectroscopy; spectroscopy; cell ... See more keywords
Light- and Redox-Dependent Force Spectroscopy Reveals that the Interaction between Plastocyanin and Plant Photosystem I Is Favored when One Partner Is Ready for Electron Transfer.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.2c06454
Abstract: Photosynthesis is a fundamental process that converts photons into chemical energy, driven by large protein complexes at the thylakoid membranes of plants, cyanobacteria, and algae. In plants, water-soluble plastocyanin (Pc) is responsible for shuttling electrons… read more here.
Keywords: psi; spectroscopy; ready electron; force spectroscopy ... See more keywords
Two-Molecule Force Spectroscopy on Proteins.
Sign Up to like & getrecommendations! Published in 2023 at "ACS nano"
DOI: 10.1021/acsnano.3c01185
Abstract: Many elastomeric proteins, which play important roles in a wide range of biological processes, exist as parallel/antiparallelly arranged dimers or multimers to perform their mechanobiological functions. For example, in striated muscle sarcomeres, the giant muscle… read more here.
Keywords: two molecule; spectroscopy; elastomeric proteins; force spectroscopy ... See more keywords
Elastin-like Polypeptide Linkers for Single-Molecule Force Spectroscopy.
Sign Up to like & getrecommendations! Published in 2017 at "ACS nano"
DOI: 10.1021/acsnano.7b02694
Abstract: Single-molecule force spectroscopy (SMFS) is by now well established as a standard technique in biophysics and mechanobiology. In recent years, the technique has benefitted greatly from new approaches to bioconjugation of proteins to surfaces. Indeed,… read more here.
Keywords: single molecule; force spectroscopy; elastin like; force ... See more keywords
Conformations and cryo-force spectroscopy of spray-deposited single-strand DNA on gold
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-08531-4
Abstract: Cryo-electron microscopy can determine the structure of biological matter in vitrified liquids. However, structure alone is insufficient to understand the function of native and engineered biomolecules. So far, their mechanical properties have mainly been probed… read more here.
Keywords: force spectroscopy; microscopy; spray deposited; dna ... See more keywords