Engineering Formaldehyde Dehydrogenase from Pseudomonas putida to Favor Nicotinamide Cytosine Dinucleotide
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202100697
Abstract: The enzyme formaldehyde dehydrogenase (FalDH) from Pseudomonas putida is of particular interest for biotechnological applications as it catalyzes the oxidation of formaldehyde independent of glutathione. However, the consumption of a stoichiometric amount of nicotinamide adenine… read more here.
Keywords: nicotinamide cytosine; favor nicotinamide; cytosine dinucleotide; pseudomonas putida ... See more keywords
Development of formaldehyde dehydrogenase‐coupled assay and antibody‐based assays for ALKBH5 activity evaluation
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2018.09.018
Abstract: &NA; N6‐methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). Until now, two RNA demethylases have been identified, including FTO (fat mass and obesity‐associated protein) and ALKBH5 (&agr;‐ketoglutarate‐dependent dioxygenase alkB homologue… read more here.
Keywords: antibody based; assays alkbh5; dehydrogenase coupled; formaldehyde dehydrogenase ... See more keywords
A direct electron transfer formaldehyde dehydrogenase biosensor for the determination of formaldehyde in river water.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122657
Abstract: In this work, we report the construction of a direct electron transfer (DET) biosensor based on NAD-dependent formaldehyde dehydrogenase from Pseudomonas sp. (FDH) immobilized on the gold nanoparticle-modified gold electrode. To the best of our… read more here.
Keywords: direct electron; biosensor; electron transfer; formaldehyde dehydrogenase ... See more keywords
Activation of the catalytic function of formaldehyde dehydrogenase for formate reduction by single-electron reduced methylviologen
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DOI: 10.1039/c8nj02211a
Abstract: The kinetic properties of formate reduction to formaldehyde with formaldehyde dehydrogenase (FldDH) using single-electron reduced methylviologen (MV˙) as a co-enzyme were clarified. By using natural co-enzyme NADH, actually, no formaldehyde production due to formate reduction… read more here.
Keywords: formate reduction; reduction; single electron; electron reduced ... See more keywords