A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2132-5_27
Abstract: Plasmodesmata (PD) are channels in the walls of plant cells which enable cell-to-cell information transfer. This includes the selective transport of specific transcription factors that control cell fate during plant development. KNOTTED1 (KN1) homeobox (KNOX)… read more here.
Keywords: forward genetic; approach; trichome rescue; genetic approach ... See more keywords
Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7262-3_17
Abstract: Subcellular targeting of vacuolar proteins depends on cellular machinery regulating vesicular trafficking. Plant-specific vacuolar trafficking routes have been reported. However, regulators mediating these processes are obscure. By combining a fluorescence imaging-based forward genetic approach and… read more here.
Keywords: vacuolar trafficking; fluorescence imaging; based forward; imaging based ... See more keywords
A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes
Sign Up to like & getrecommendations! Published in 2019 at "Autophagy"
DOI: 10.1080/15548627.2019.1569915
Abstract: ABSTRACT Macroautophagy is a process through which eukaryotic cells degrade large substrates including organelles, protein aggregates, and invading pathogens. Over 40 autophagy-related (ATG) genes have been identified through forward-genetic screens in yeast. Although homology-based analyses… read more here.
Keywords: screen; autophagy; facile forward; arabidopsis ... See more keywords
Marker-assisted mapping enables forward genetic analysis in Aedes aegypti, an arboviral vector with vast recombination deserts.
Sign Up to like & getrecommendations! Published in 2022 at "Genetics"
DOI: 10.1093/genetics/iyac140
Abstract: Aedes aegypti is a major vector of arboviruses that cause dengue, chikungunya, yellow fever and Zika. Although recent success in reverse genetics has facilitated rapid progress in basic and applied research, integration of forward genetics… read more here.
Keywords: aedes aegypti; recombination; marker assisted; vector ... See more keywords
SLiM 2: Flexible, Interactive Forward Genetic Simulations
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biology and Evolution"
DOI: 10.1093/molbev/msw211
Abstract: Modern population genomic datasets hold immense promise for revealing the evolutionary processes operating in natural populations, but a crucial prerequisite for this goal is the ability to model realistic evolutionary scenarios and predict their expected… read more here.
Keywords: flexible interactive; genetic simulations; slim flexible; evolutionary scenarios ... See more keywords
A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5′ splice site identity
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac991
Abstract: Abstract The spliceosome undergoes extensive rearrangements as it assembles onto precursor messenger RNAs. In the earliest assembly step, U1snRNA identifies the 5′ splice site. However, U1snRNA leaves the spliceosome relatively early in assembly, and 5′… read more here.
Keywords: snrnp200 brr2; genetic screen; forward genetic; site identity ... See more keywords
Novel cancer gene discovery using a forward genetic screen in RCAS-PDGFB-driven gliomas
Sign Up to like & getrecommendations! Published in 2022 at "Neuro-Oncology"
DOI: 10.1093/neuonc/noac158
Abstract: Abstract Background Malignant gliomas, the most common malignant brain tumors in adults, represent a heterogeneous group of diseases with poor prognosis. Retroviruses can cause permanent genetic alterations that modify genes close to the viral integration… read more here.
Keywords: rcas; rcas pdgfb; forward genetic; pdgfb driven ... See more keywords
Unbiased proteomic and forward genetic screens reveal that mechanosensitive ion channel MSL10 functions at ER–plasma membrane contact sites in Arabidopsis thaliana
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2022.05.23.493056
Abstract: Mechanosensitive (MS) ion channels are an evolutionarily conserved way for cells to sense mechanical forces and transduce them into ionic signals. The channel properties of Arabidopsis thaliana MscS-Like (MSL)10 have been well studied, but how… read more here.
Keywords: membrane contact; mechanosensitive ion; forward genetic; msl10 ... See more keywords
Abstract 5773: A forward genetic screen identifies SIRT1 as a driver of neuroendocrine prostate cancer
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-5773
Abstract: Neuroendocrine prostate cancer (NEPC) is a deadly variant of prostate cancer for which no cure is yet available. Up to 20 percent of the patients with castration resistant prostate cancer (CRPC), will progress to NEPC… read more here.
Keywords: prostate cancer; prostate; neuroendocrine prostate; mouse ... See more keywords
A forward genetic screen identifies Dolk as a regulator of startle magnitude through the potassium channel subunit Kv1.1
Sign Up to like & getrecommendations! Published in 2021 at "PLoS Genetics"
DOI: 10.1371/journal.pgen.1008943
Abstract: The acoustic startle response is an evolutionarily conserved avoidance behavior. Disruptions in startle behavior, particularly startle magnitude, are a hallmark of several human neurological disorders. While the neural circuitry underlying startle behavior has been studied… read more here.
Keywords: magnitude; startle behavior; channel subunit; forward genetic ... See more keywords
A forward genetic screen reveals a primary role for Plasmodium falciparum Reticulocyte Binding Protein Homologue 2a and 2b in determining alternative erythrocyte invasion pathways
Sign Up to like & getrecommendations! Published in 2018 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1007436
Abstract: Invasion of human erythrocytes is essential for Plasmodium falciparum parasite survival and pathogenesis, and is also a complex phenotype. While some later steps in invasion appear to be invariant and essential, the earlier steps of… read more here.
Keywords: invasion; invasion pathways; plasmodium falciparum; role ... See more keywords