CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae
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DOI: 10.1002/jobm.201800195
Abstract: In this study, we focused on the applicability of CRISPR/Cpf1 in genome simplification of Saccharomyces cerevisiae and established a CRISPR/Cpf1 assisted method for rapid markerless large fragment deletion to facilitate laboratory evolution of geome of… read more here.
Keywords: fragment deletion; crispr cpf1; saccharomyces cerevisiae; large fragment ... See more keywords