Efficient assembly of a large fragment of monkeypox virus genome as a qPCR template using dual-selection based transformation-associated recombination.
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DOI: 10.1016/j.virs.2022.02.009
Abstract: Transformation-associated recombination (TAR) has been widely used to assemble large DNA constructs. One of the significant obstacles hindering assembly efficiency is the presence of error-prone DNA repair pathways in yeast, which results in vector backbone… read more here.
Keywords: fragment monkeypox; transformation associated; selection; tar ... See more keywords