A Review of Methodologies for the Detection, Quantitation, and Localization of Free Cysteine in Recombinant Proteins: A Focus on Therapeutic Monoclonal Antibodies
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DOI: 10.3389/fmolb.2022.886417
Abstract: Free-cysteine residues in recombinant biotherapeutics such as monoclonal antibodies can arise from incorrect cellular processing of disulfide bonds during synthesis or by reduction of disulfide bonds during the harvest and purification stage of manufacture. Free… read more here.
Keywords: free cysteine; methodologies detection; review methodologies; free cysteines ... See more keywords
The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
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DOI: 10.7717/peerj.13923
Abstract: Background Tuna muscle greening is a problem that occurs after heating. A hypothesis has been postulated to address this problem, involving a conserved Cys residue at position 10 (Cys-10) present on tuna myoglobin (Mb) that… read more here.
Keywords: thermal treatment; free cysteine; reaction; cys ... See more keywords