Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput
Sign Up to like & getrecommendations! Published in 2021 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.0c00538
Abstract: Compartmentalization of single genes in water-in-oil emulsion droplets is a powerful approach to create millions of reactors for enzyme library selections. When these droplets are formed at ultrahigh throughput in microfluidic devices, their perfect monodispersity… read more here.
Keywords: free directed; evolution; protease; cell free ... See more keywords
Metal-free directed C-H bond activation and borylation.
Sign Up to like & getrecommendations! Published in 2019 at "Nature"
DOI: 10.1038/s41586-019-1640-2
Abstract: Organoboron reagents are important synthetic intermediates that play a key role in the construction of natural products, pharmaceuticals, and organic materials.1 The discovery of simpler, milder and more efficient approaches to organoborons opens a route… read more here.
Keywords: free directed; directed bond; borylation; activation ... See more keywords