An improved digital polymerase chain reaction protocol to capture low‐copy KRAS mutations in plasma cell‐free DNA by resolving ‘subsampling’ issues
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12110
Abstract: Genetic alterations responsible for the initiation of cancer may serve as immediate biomarkers for early diagnosis. Plasma levels of cell‐free DNA (cfDNA) in patients with cancer are higher than those in healthy individuals; however, the… read more here.
Keywords: resolving subsampling; free dna; cell free; chain reaction ... See more keywords
Clinical significance of circulating tumor cells and cell‐free DNA in pediatric rhabdomyosarcoma
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13197
Abstract: Liquid biopsy analysis represents a powerful and noninvasive tool to uncover biomarkers for disseminated disease assessment and longitudinal monitoring of patients. Herein, we explored the value of circulating and disseminated tumor cells (CTC and DTC,… read more here.
Keywords: cell free; free dna; tumor cells; pediatric rhabdomyosarcoma ... See more keywords
Engineered Microneedles for Interstitial Fluid Cell‐Free DNA Capture and Sensing Using Iontophoretic Dual‐Extraction Wearable Patch
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202000591
Abstract: Interstitial fluid (ISF), as an emerging source of biomarkers, is unmistakably significant for disease diagnosis. Microneedles (MNs) provide a minimally invasive approach for extracting the desired molecules from ISF. However, existing MNs are limited by… read more here.
Keywords: free dna; capture; interstitial fluid; cell free ... See more keywords
A Cell‐free DNA Barcode‐Enabled Single‐Molecule Test for Noninvasive Prenatal Diagnosis of Monogenic Disorders: Application to β‐Thalassemia
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Science"
DOI: 10.1002/advs.201802332
Abstract: Abstract Noninvasive prenatal testing of common aneuploidies has become routine over the past decade, but testing of monogenic disorders remains a challenge in clinical implementation. Most recent studies have inherent limitations, such as complicated procedures,… read more here.
Keywords: molecule; monogenic disorders; barcode enabled; free dna ... See more keywords
Cell‐free DNA analysis for detection of MYD88L265P and CXCR4S338X mutations in Waldenström macroglobulinemia
Sign Up to like & getrecommendations! Published in 2021 at "American Journal of Hematology"
DOI: 10.1002/ajh.26184
Abstract: MYD88L265P and CXCR4S338X variants are highly prevalent and impact disease presentation, prognosis, and/or treatment outcome in Waldenstrom's Macroglobulinemia (WM). The use of bone marrow (BM) aspirate represents the current "gold standard" for molecular testing in… read more here.
Keywords: free dna; cell free; cxcr4s338x mutations; myd88l265p cxcr4s338x ... See more keywords
A field guide for cancer diagnostics using cell‐free DNA: From principles to practice and clinical applications
Sign Up to like & getrecommendations! Published in 2018 at "Genes"
DOI: 10.1002/gcc.22517
Abstract: Recently, many genome‐wide profiling studies provided insights into the molecular make‐up of major cancer types. The deeper understanding of these genetic alterations and their functional consequences led to the discovery of novel therapeutic opportunities improving… read more here.
Keywords: dna; free dna; cell free; clinical applications ... See more keywords
Noninvasive detection of brain gliomas using plasma cell‐free DNA 5‐hydroxymethylcytosine sequencing
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Cancer"
DOI: 10.1002/ijc.34401
Abstract: Liquid biopsy techniques based on deep sequencing of plasma cell‐free DNA (cfDNA) could detect the low‐frequency somatic mutations and provide an accurate diagnosis for many cancers. However, for brain gliomas, reliable performance of these techniques… read more here.
Keywords: brain gliomas; cell free; plasma cell; gliomas ... See more keywords
Noninvasive prenatal diagnosis based on cell-free DNA for tuberous sclerosis: A pilot study.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular genetics & genomic medicine"
DOI: 10.1002/mgg3.1952
Abstract: BACKGROUND Noninvasive prenatal diagnosis (NIPD) based on cell-free DNA (cfDNA) has been introduced into the clinical application for some monogenic disorders but not for tuberous sclerosis (TSC) yet, which is an autosomal dominant disease caused… read more here.
Keywords: dna; cell free; prenatal diagnosis; based cell ... See more keywords
Comparison of first‐tier cell‐free DNA screening for common aneuploidies with conventional publically funded screening
Sign Up to like & getrecommendations! Published in 2017 at "Prenatal Diagnosis"
DOI: 10.1002/pd.5174
Abstract: This study evaluates the impact of offering cell‐free DNA (cfDNA) screening as a first‐tier test for trisomies 21 and 18. read more here.
Keywords: first tier; free dna; cell free; tier cell ... See more keywords
Current controversies in prenatal diagnosis 2: Cell‐free DNA prenatal screening should be used to identify all chromosome abnormalities
Sign Up to like & getrecommendations! Published in 2018 at "Prenatal Diagnosis"
DOI: 10.1002/pd.5216
Abstract: Noninvasive prenatal testing (NIPT) using cell‐free DNA (cfDNA) from maternal serum has been clinically available since 2011. This technology has revolutionized our ability to screen for the common aneuploidies trisomy 21 (Down syndrome), trisomy 18,… read more here.
Keywords: prenatal screening; chromosome abnormalities; free dna; cell free ... See more keywords
Antenatal screening for fetal trisomies using microarray‐based cell‐free DNA testing: A systematic review and meta‐analysis
Sign Up to like & getrecommendations! Published in 2019 at "Prenatal Diagnosis"
DOI: 10.1002/pd.5621
Abstract: To evaluate the test accuracy of non‐invasive prenatal testing (NIPT) for fetal trisomy 21, 18, and 13 using cell‐free (cf) DNA analysis in maternal plasma with microarray quantitation. read more here.
Keywords: antenatal screening; microarray; analysis; free dna ... See more keywords