An improved digital polymerase chain reaction protocol to capture low‐copy KRAS mutations in plasma cell‐free DNA by resolving ‘subsampling’ issues
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12110
Abstract: Genetic alterations responsible for the initiation of cancer may serve as immediate biomarkers for early diagnosis. Plasma levels of cell‐free DNA (cfDNA) in patients with cancer are higher than those in healthy individuals; however, the… read more here.
Keywords: resolving subsampling; free dna; cell free; chain reaction ... See more keywords
Clinical significance of circulating tumor cells and cell‐free DNA in pediatric rhabdomyosarcoma
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13197
Abstract: Liquid biopsy analysis represents a powerful and noninvasive tool to uncover biomarkers for disseminated disease assessment and longitudinal monitoring of patients. Herein, we explored the value of circulating and disseminated tumor cells (CTC and DTC,… read more here.
Keywords: cell free; free dna; tumor cells; pediatric rhabdomyosarcoma ... See more keywords
Engineered Microneedles for Interstitial Fluid Cell‐Free DNA Capture and Sensing Using Iontophoretic Dual‐Extraction Wearable Patch
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202000591
Abstract: Interstitial fluid (ISF), as an emerging source of biomarkers, is unmistakably significant for disease diagnosis. Microneedles (MNs) provide a minimally invasive approach for extracting the desired molecules from ISF. However, existing MNs are limited by… read more here.
Keywords: free dna; capture; interstitial fluid; cell free ... See more keywords
Pathogenesis Activated “In Situ Disassembly‐Reassembly” Pattern Dual‐Scavenges Cell‐Free DNA/ROS for Hemorrhagic Stroke Management
Sign Up to like & getrecommendations! Published in 2025 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202424358
Abstract: Intracerebral hemorrhage (ICH) causes uncontrolled neuroinflammation/oxidative stress to aggravate secondary brain injury (SBI) for high mortality and disability but lacks effective pharmacotherapy in clinical practices. This represents an imperative need to discover potential targets and… read more here.
Keywords: free dna; cell free; disassembly reassembly; cfdna ... See more keywords
A Cell‐free DNA Barcode‐Enabled Single‐Molecule Test for Noninvasive Prenatal Diagnosis of Monogenic Disorders: Application to β‐Thalassemia
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Science"
DOI: 10.1002/advs.201802332
Abstract: Abstract Noninvasive prenatal testing of common aneuploidies has become routine over the past decade, but testing of monogenic disorders remains a challenge in clinical implementation. Most recent studies have inherent limitations, such as complicated procedures,… read more here.
Keywords: molecule; monogenic disorders; barcode enabled; free dna ... See more keywords
Cell‐free DNA analysis for detection of MYD88L265P and CXCR4S338X mutations in Waldenström macroglobulinemia
Sign Up to like & getrecommendations! Published in 2021 at "American Journal of Hematology"
DOI: 10.1002/ajh.26184
Abstract: MYD88L265P and CXCR4S338X variants are highly prevalent and impact disease presentation, prognosis, and/or treatment outcome in Waldenstrom's Macroglobulinemia (WM). The use of bone marrow (BM) aspirate represents the current "gold standard" for molecular testing in… read more here.
Keywords: free dna; cell free; cxcr4s338x mutations; myd88l265p cxcr4s338x ... See more keywords
Leveraging archival cerebrospinal fluid samples for genetic insights from cell‐free DNA
Sign Up to like & getrecommendations! Published in 2024 at "Cancer Cytopathology"
DOI: 10.1002/cncy.22794
Abstract: Cerebrospinal fluid (CSF) samples are often a rich source of tumor‐derived cell‐free DNA (cfDNA), a high degree of success in detecting tumor mutations can even be achieved with archival CSF samples. read more here.
Keywords: cerebrospinal fluid; free dna; leveraging archival; cell free ... See more keywords
A field guide for cancer diagnostics using cell‐free DNA: From principles to practice and clinical applications
Sign Up to like & getrecommendations! Published in 2018 at "Genes"
DOI: 10.1002/gcc.22517
Abstract: Recently, many genome‐wide profiling studies provided insights into the molecular make‐up of major cancer types. The deeper understanding of these genetic alterations and their functional consequences led to the discovery of novel therapeutic opportunities improving… read more here.
Keywords: dna; free dna; cell free; clinical applications ... See more keywords
Noninvasive detection of brain gliomas using plasma cell‐free DNA 5‐hydroxymethylcytosine sequencing
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Cancer"
DOI: 10.1002/ijc.34401
Abstract: Liquid biopsy techniques based on deep sequencing of plasma cell‐free DNA (cfDNA) could detect the low‐frequency somatic mutations and provide an accurate diagnosis for many cancers. However, for brain gliomas, reliable performance of these techniques… read more here.
Keywords: brain gliomas; cell free; plasma cell; gliomas ... See more keywords
Harnessing viral footprints in circulating free DNA (cfDNA) for early cancer detection: A focus on liquid‐biopsy‐based screening
Sign Up to like & getrecommendations! Published in 2025 at "International Journal of Cancer"
DOI: 10.1002/ijc.70121
Abstract: Viral infections play a significant role in cancer development, making detecting viral signatures a promising approach for early cancer diagnosis. Circulating free DNA (cfDNA), released into the bloodstream by tumors and other cells, has emerged… read more here.
Keywords: cancer; free dna; cfdna; early cancer ... See more keywords
Noninvasive prenatal diagnosis based on cell-free DNA for tuberous sclerosis: A pilot study.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular genetics & genomic medicine"
DOI: 10.1002/mgg3.1952
Abstract: BACKGROUND Noninvasive prenatal diagnosis (NIPD) based on cell-free DNA (cfDNA) has been introduced into the clinical application for some monogenic disorders but not for tuberous sclerosis (TSC) yet, which is an autosomal dominant disease caused… read more here.
Keywords: dna; cell free; prenatal diagnosis; based cell ... See more keywords