Comparison of antimicrobial peptide purification via free‐flow electrophoresis and gel filtration chromatography
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700187
Abstract: Antimicrobial peptides (AMPs) are usually small and cationic biomolecules with broad‐spectrum antimicrobial activities against pathogens. Purifying them from complex samples is essential to study their physiochemical properties. In this work, free‐flow zone electrophoresis (FFZE) was… read more here.
Keywords: electrophoresis; filtration chromatography; free flow; gel filtration ... See more keywords
Reduced surface adsorption in 3D printed acrylonitrile butadiene styrene micro free‐flow electrophoresis devices
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201900179
Abstract: We have 3D printed and fabricated micro free‐flow electrophoresis (µFFE) devices in acrylonitrile butadiene styrene (ABS) that exhibit minimal surface adsorption without requiring additional surface coatings or specialized buffer additives. 2D, nano LC–micro free flow… read more here.
Keywords: surface adsorption; adsorption; flow electrophoresis; free flow ... See more keywords
Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology
Sign Up to like & getrecommendations! Published in 2020 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202000149
Abstract: The increasing resolution of three‐dimensional (3D) printing offers simplified access to, and development of, microfluidic devices with complex 3D structures. Therefore, this technology is increasingly used for rapid prototyping in laboratories and industry. Microfluidic free… read more here.
Keywords: production; electrophoresis; flow electrophoresis; free flow ... See more keywords
Investigating peak dispersion in free‐flow counterflow gradient focusing due to electroosmotic flow
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200230
Abstract: Free‐flow electrophoresis (FFE) has the ability to continuously separate charged solutes from complex biological mixtures. Recently, a free‐flow counterflow gradient focusing mechanism has been introduced to FFE, and it offers the potential for improved resolution… read more here.
Keywords: gradient focusing; flow counterflow; free flow; flow ... See more keywords
Micro free‐flow isoelectric focusing with integrated optical pH sensors
Sign Up to like & getrecommendations! Published in 2018 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201700035
Abstract: Recently, a new observation method for monitoring of pH gradients in microfluidic free‐flow electrophoresis has emerged. It is based on the use of chip‐integrated fluorescent or luminescent micro sensor layers. These are able to monitor… read more here.
Keywords: focusing integrated; free flow; micro free; flow isoelectric ... See more keywords
Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7389-7_8
Abstract: Free-flow electrophoresis (FFE) is a technique for separation of proteins, peptides, organelles, and cells. With zone electrophoresis (ZE-FFE), organelles are separated according to surface charge. The ER is the only remaining major cellular compartment in… read more here.
Keywords: surface charge; electrophoresis; flow electrophoresis; free flow ... See more keywords
Superconvergence of MAC Scheme for a Coupled Free Flow-Porous Media System with Heat Transport on Non-uniform Grids
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Scientific Computing"
DOI: 10.1007/s10915-022-01763-5
Abstract: In this paper, a marker and cell (MAC) scheme is constructed for the free flow-porous media system with heat transport on non-uniform grids, where the Stokes-Darcy equation is employed to describe the free-flow and the… read more here.
Keywords: flow porous; free flow; uniform grids; non uniform ... See more keywords
Current advances and challenges in microfluidic free-flow electrophoresis-A critical review.
Sign Up to like & getrecommendations! Published in 2017 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2017.08.017
Abstract: The research field on microfluidic free-flow electrophoresis has developed vast amounts of devices, methods, applications and raised new questions, often in analogy to conventional techniques from which it derives. Most efforts have been employed on… read more here.
Keywords: flow electrophoresis; free flow; review; microfluidic free ... See more keywords
Design and analysis of 0.127 m (5″) Cutthroat flume
Sign Up to like & getrecommendations! Published in 2017 at "Ain Shams Engineering Journal"
DOI: 10.1016/j.asej.2015.07.017
Abstract: Abstract The aim of the present work was to perform series of detailed laboratory experiments under steady-state flow conditions through a Cutthroat flume of throat width 0.127 m (5″) and length 0.918 m. It is very important… read more here.
Keywords: throat width; cutthroat flume; free flow; flow submerged ... See more keywords
Free-flow discharge estimation method for Piano Key weir geometries
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Hydro-environment Research"
DOI: 10.1016/j.jher.2017.10.003
Abstract: Abstract Currently, many existing spillways located throughout the globe require rehabilitation due to dam safety issues. Consequently, there is an increasing need for design tools that facilitate better, more economical spillway designs. Piano Key weirs… read more here.
Keywords: method; discharge; free flow; piano key ... See more keywords
Characterization of the acidic species of a monoclonal antibody using free flow electrophoresis fractionation and mass spectrometry.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113217
Abstract: Antibody charge heterogeneity is one of the major product-related variants in recombinant biopharmaceuticals, which has been commonly monitored by imaged capillary isoelectric focusing (icIEF). Due to the challenges with sample recovery and fractionation, other charge-based… read more here.
Keywords: fractionation; antibody; flow electrophoresis; free flow ... See more keywords