Branched DNA Architectures Produced by PCR‐Based Assembly as Gene Compartments for Cell‐Free Gene‐Expression Reactions
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201900094
Abstract: The physical distance between genes plays important roles in controlling gene expression reactions in vivo. Herein, we report the design and synthesis of a branched gene architecture in which three transcription units are integrated into… read more here.
Keywords: free gene; gene expression; cell free; expression reactions ... See more keywords
Efficient production of transgene-free, gene-edited carrot plants via protoplast transformation.
Sign Up to like & getrecommendations! Published in 2022 at "Plant cell reports"
DOI: 10.1007/s00299-022-02830-9
Abstract: KEY MESSAGE We have developed and validated an efficient protocol for producing gene-edited carrot plants that do not result in the stable incorporation of foreign DNA in the edited plant's genome. We report here a… read more here.
Keywords: gene edited; edited carrot; carrot plants; transgene free ... See more keywords
A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00051
Abstract: Non-canonical amino acids (ncAAs) can be incorporated into peptides and proteins to create new properties and functions. Site-specific ncAA incorporation is typically enabled by orthogonal translation systems comprising a stop codon suppressing tRNA (typically UAG),… read more here.
Keywords: stop codon; cell free; codon suppressing; free gene ... See more keywords
Cell-Free Gene Expression Dynamics in Synthetic Cell Populations
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.1c00376
Abstract: The ability to build synthetic cellular populations from the bottom-up provides the groundwork to realize minimal living tissues comprising single cells which can communicate and bridge scales into multicellular systems. Engineered systems made of synthetic… read more here.
Keywords: cell free; expression; free gene; gene expression ... See more keywords
Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00253
Abstract: Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw… read more here.
Keywords: free gene; biology; gene expression; cell free ... See more keywords
Cell-free gene expression: an expanded repertoire of applications
Sign Up to like & getrecommendations! Published in 2019 at "Nature Reviews Genetics"
DOI: 10.1038/s41576-019-0186-3
Abstract: Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a… read more here.
Keywords: free gene; biology; gene expression; cell free ... See more keywords
Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform
Sign Up to like & getrecommendations! Published in 2022 at "Synthetic Biology"
DOI: 10.1093/synbio/ysac008
Abstract: Abstract Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established… read more here.
Keywords: cell; cell free; expression; free gene ... See more keywords
A simple and efficient strategy to produce transgene-free gene edited plants in one generation using paraquat resistant 1 as a selection marker
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.1051991
Abstract: Introduction DNA integration is a key factor limiting the marketing of CRISPR/Cas9-mediated gene edited crops. Several strategies have been established to obtain transgene-free gene edited plants; however, these strategies are usually time-consuming, technically difficult, providing… read more here.
Keywords: edited plants; free gene; strategy; gene edited ... See more keywords