Transcriptomic Insights Into Serum‐Free Medium Adaptation and Temperature Reduction in Chinese Hamster Ovary Cell Cultures
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DOI: 10.1002/biot.70055
Abstract: Chinese hamster ovary (CHO) cells are widely used in recombinant biopharmaceutical production; yet, yields remain low, leading to high market prices. Improving product yield and quality has heavily relied on empirical characterization with limited insight… read more here.
Keywords: temperature; adaptation; serum free; free medium ... See more keywords
A serum-free medium suitable for maintaining cell morphology and liver-specific function in induced human hepatocytes
Sign Up to like & getrecommendations! Published in 2018 at "Cytotechnology"
DOI: 10.1007/s10616-018-0289-2
Abstract: AbstracthiHep is a new type of hepatocyte-like cell that is predicted to be a potential unlimited source of hepatocytes for a bioartificial liver. However, hiHep cannot currently be used in clinical settings because serum must… read more here.
Keywords: liver specific; medium; free medium; serum free ... See more keywords
Efficient oxidative esterification of furfural to methylfuroate over Au/Al2O3 catalyst in base-free medium
Sign Up to like & getrecommendations! Published in 2024 at "New Journal of Chemistry"
DOI: 10.1039/d3nj05231a
Abstract: Oxidative esterification of furfural to methylfuroate was proceeded in base-free medium over Au catalysts supported on ZrO2, CeO2, TiO2, ZnO and Al2O3, and Au/Al2O3 catalyst exhibited higher activity than the... read more here.
Keywords: furfural methylfuroate; free medium; al2o3 catalyst; base free ... See more keywords
A new clinical‐scale serum‐free xeno‐free medium efficient in ex vivo amplification of mesenchymal stromal cells does not support mesenchymal stem cells
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DOI: 10.1111/trf.13902
Abstract: We evaluated a new serum‐free, xeno‐free medium (Xuri, GE HealthCare) in ex vivo cultures for amplification of mesenchymal stromal cells (MStroC) in comparison with classical culture supplemented with fetal calf serum and basic fibroblast growth… read more here.
Keywords: mesenchymal stromal; free medium; free xeno; xeno free ... See more keywords
Efficacy of Methionine Restriction and the PARP-inhibitor Olaparib and Their Combination on BRCA1 Mutant and Wild-type Triple-negative Breast Cancer Cell Lines
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DOI: 10.21873/anticanres.17522
Abstract: Background/Aim: Triple-negative breast cancer (TNBC) is a recalcitrant disease. The present study examined the efficacy of methionine restriction and the poly ADP-ribose polymerase (PARP)-inhibitor olaparib on BRCA1/2 wild-type and BRCA1 mutated TNBC cell lines. Materials… read more here.
Keywords: free medium; brca1; cell; wild type ... See more keywords
Development of a chemically disclosed serum-free medium for mouse pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2024 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2024.1390386
Abstract: Mouse embryonic stem cells (mESCs) have been widely used as a model system to study the basic biology of pluripotency and to develop cell-based therapies. Traditionally, mESCs have been cultured in a medium supplemented with… read more here.
Keywords: serum free; free medium; pluripotent stem; stem cells ... See more keywords
Generating Bovine Monocyte-Derived Dendritic Cells for Experimental and Clinical Applications Using Commercially Available Serum-Free Medium
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2020.591185
Abstract: Advances in fundamental and applied immunology research often originate from pilot studies utilizing animal models. While cattle represent an ideal model for disease pathogenesis and vaccinology research for a number of human disease, optimized bovine… read more here.
Keywords: modc; free medium; bovine; serum free ... See more keywords
Cytokinin Potentials on In Vitro Shoot Proliferation and Subsequent Rooting of Agave sisalana Perr. Syn
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DOI: 10.3390/horticulturae11080929
Abstract: Agave species are plants with great economic value and multiple possibilities of use as ornamentals, medicinal plants, and fibers, as well as being significant sources of bioethanol. However, their long life cycles hinder their conventional… read more here.
Keywords: free medium; shoot proliferation; subsequent rooting; proliferation ... See more keywords
A Newly Developed Chemically Defined Serum-Free Medium Suitable for Human Primary Keratinocyte Culture and Tissue Engineering Applications
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DOI: 10.3390/ijms24031821
Abstract: In our experience, keratinocytes cultured in feeder-free conditions and in commercially available defined and serum-free media cannot be as efficiently massively expanded as their counterparts grown in conventional bovine serum-containing medium, nor can they properly… read more here.
Keywords: free medium; serum free; medium; defined serum ... See more keywords
Taurine Biosynthesis in a Fish Liver Cell Line (ZFL) Adapted to a Serum-Free Medium
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DOI: 10.3390/md15060147
Abstract: Although taurine has been shown to play multiple important physiological roles in teleosts, little is known about the molecular mechanisms underlying dietary requirements. Cell lines can provide useful tools for deciphering biosynthetic pathways and their… read more here.
Keywords: taurine biosynthesis; cell line; medium; free medium ... See more keywords
Novel sericin-based hepatocyte serum-free medium and sericin’s effect on hepatocyte transcriptome
Sign Up to like & getrecommendations! Published in 2018 at "World Journal of Gastroenterology"
DOI: 10.3748/wjg.v24.i30.3398
Abstract: AIM To develop a novel hepatocyte serum-free medium based on sericin, and to explore the effect of sericin on the hepatocyte transcriptome. METHODS A controlled trial comparing novel serum-free medium and other media: C3A cells… read more here.
Keywords: medium; free medium; sericin; effect ... See more keywords