Cell‐Free Protein Synthesis: A Cell‐Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo (Adv. Sci. 17/2020)
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.202070096
Abstract: In article number 2001616, Tiangang Liu and co-workers develop a cell-free protein synthesis (CFPS) platform that enables rapid discovery of novel lanthipeptides and guides lanthipeptide overproduction in vivo. This study extends the use of cell-free… read more here.
Keywords: cell free; protein synthesis; novel lanthipeptides; cell ... See more keywords
Cell‐Free Protein Synthesis for the Screening of Novel Azoreductases and Their Preferred Electron Donor
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200121
Abstract: Azoreductases are potent biocatalysts for the cleavage of azo bonds. Various gene sequences coding for potential azoreductases are available in databases, but many of their gene products are still uncharacterized. To avoid the laborious heterologous… read more here.
Keywords: preferred electron; electron donor; cell free; free protein ... See more keywords
Fungal insecticidal activity elevated by non-risky markerless overexpression of an endogenous cysteine-free protein gene in Beauveria bassiana.
Sign Up to like & getrecommendations! Published in 2022 at "Pest management science"
DOI: 10.1002/ps.6946
Abstract: BACKGROUND Fungal insecticides are notorious for slow kill action, an intrinsic trait that can be improved by genetic engineering of an exogenous or endogenous virulence factor. However, transgenic insecticides expressing exogenous toxin and herbicide-resistant marker… read more here.
Keywords: free protein; fungal insecticidal; activity; gene ... See more keywords
Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0524-0_11
Abstract: Cell-free protein synthesis (CFPS) is an established method to produce recombinant proteins and has been used in a wide variety of applications. The use of CFPS has almost from the onset been favorably linked to… read more here.
Keywords: nmr spectroscopy; cell free; proteins nmr; protein synthesis ... See more keywords
A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties.
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DOI: 10.1007/978-1-4939-9121-1_4
Abstract: Fluorescent labeling of de novo synthesized proteins is in particular a valuable tool for functional and structural studies of membrane proteins. In this context, we present two methods for the site-specific fluorescent labeling of difficult-to-express… read more here.
Keywords: protein; cell free; protein synthesis; fluorescent labeling ... See more keywords
Regulatory-sequence mechanical biosensor: A versatile platform for investigation of G-quadruplex/label-free protein interactions and tunable protein detection.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.09.019
Abstract: Mechanical biosensors can be used to quantitatively explore DNA-protein binding mechanisms by detecting targets at low concentrations or measuring force in single-molecule force spectroscopy. However, restrictions in single-molecule manipulation and labelling protocols have hindered the… read more here.
Keywords: protein; detection; force; label free ... See more keywords
Cell-free protein synthesis for producing ‘difficult-to-express’ proteins
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2018.07.013
Abstract: Abstract Biomanufacturing requires the stable production of active biological molecules including proteins. However, a significant percentage of genes cannot be expressed efficiently in a cell-based system due to cellular homeostasis. Upon overproduction, proteins often form… read more here.
Keywords: production; cell free; express proteins; free protein ... See more keywords
Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2018.05.005
Abstract: Since phosphorylation is involved in various physiological events, kinases and interacting factors can be potential targets for drug discovery. For the development and improvement of inhibitors from the point of view of mechanistic enzymology, a… read more here.
Keywords: protein; protein expression; kinase; cell free ... See more keywords
Cell-Free Protein Synthesis Approach to Biosensing hTRβ-Specific Endocrine Disruptors.
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DOI: 10.1021/acs.analchem.6b04034
Abstract: Here we introduce a Rapid Adaptable Portable In vitro Detection biosensor platform (RAPID) for detecting ligands that interact with nuclear hormone receptors (NHRs). The RAPID platform can be adapted for field use, allowing rapid evaluation… read more here.
Keywords: protein; biosensor; cell free; protein synthesis ... See more keywords
A Cell-Free Protein Expression System Derived from Human Primary Peripheral Blood Mononuclear Cells.
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DOI: 10.1021/acssynbio.0c00256
Abstract: Historically, some of the first cell-free protein expression systems studied in-vitro translation in various human blood cells. However because of limited knowledge of eukaryotic translation and the advancement of cell line development, interest in these… read more here.
Keywords: cell free; translation; biology; free protein ... See more keywords
In Vitro Synthesis and Design of Kinesin Biomolecular Motors by Cell-Free Protein Synthesis.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.3c00235
Abstract: Kinesin is a biomolecular motor that generates force and motility along microtubule cytoskeletons in cells. Owing to their ability to manipulate cellular nanoscale components, microtubule/kinesin systems show great promise as actuators of nanodevices. However, classical… read more here.
Keywords: cell free; synthesis; kinesin biomolecular; free protein ... See more keywords