Short DNA containing χ sites enhances DNA stability and gene expression in E. coli cell-free transcription-translation systems.
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.26333
Abstract: Escherichia coli cell-free transcription-translation (TXTL) systems offer versatile platforms for advanced biomanufacturing and for prototyping synthetic biological parts and devices. Production and testing could be accelerated with the use of linear DNA, which can be… read more here.
Keywords: dna; free transcription; cell free; expression ... See more keywords
A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of biotechnology"
DOI: 10.1016/j.btecx.2020.100024
Abstract: Cell-free transcription-translation systems are a versatile tool to study gene expression, enzymatic reactions and biochemical regulation mechanisms. Because cell-free transcription-translation systems are often derived from cell lysates, many different substances, among them amino acids, are… read more here.
Keywords: transcription translation; free transcription; lysine; cell free ... See more keywords
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.12.007
Abstract: CRISPR-Cas systems offer versatile technologies for genome engineering, yet their implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti-CRISPR proteins. Here, we present the use of E. coli cell-free transcription-translation (TXTL) systems… read more here.
Keywords: transcription translation; crispr; free transcription; cell free ... See more keywords
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.05.014
Abstract: The characterization of CRISPR-Cas immune systems in bacteria was quickly followed by the discovery of anti-CRISPR proteins (Acrs) in bacteriophages. These proteins block different steps of CRISPR-based immunity and, as some inhibit Cas nucleases, can… read more here.
Keywords: transcription translation; free transcription; cell free; translation system ... See more keywords
Cell-Free Transcription-Coupled CRISPR/Cas12a Assay for Prototyping Cyanobacterial Promoters.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00148
Abstract: Cyanobacteria are promising microbial hosts for the production of diverse biofuels and biochemicals. However, compared to other model microbial hosts such as Escherichia coli and yeast, it takes a long time to genetically modify cyanobacteria.… read more here.
Keywords: crispr cas12a; transcription; free transcription; cell free ... See more keywords