Effects of Charged Lipids on Giant Unilamellar Vesicle Fusion and Inner Content Mixing via Freeze‐Thawing
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DOI: 10.1002/cbic.202200550
Abstract: Fusion between giant unilamellar vesicles (GUVs) can incorporate and mix components of biochemical reactions. Recently, GUV fusion induced by freeze‐thawing (F/T) was employed to construct artificial cells that can easily and repeatedly fuse GUVs with… read more here.
Keywords: fusion; content mixing; effects charged; charged lipids ... See more keywords
Study of the mechanical properties of fresh and cryopreserved individual human oocytes
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DOI: 10.1007/s00249-019-01379-y
Abstract: In assisted reproduction technologies, the cryopreservation of oocytes is a common procedure used to circumvent female infertility. However, some morphological and functional alterations of oocytes have been observed depending on the protocol applied. In this… read more here.
Keywords: freeze thawing; microscopy; human oocytes; individual human ... See more keywords
Characteristics of some physically modified starches using mild heating and freeze-thawing
Sign Up to like & getrecommendations! Published in 2017 at "Food Hydrocolloids"
DOI: 10.1016/j.foodhyd.2017.11.035
Abstract: Abstract Granular starches of different origins (normal corn, waxy corn, potato and tapioca) dispersed in water (40% dry solids, w/v) were subjected to mild heating (1 h) at their onset temperatures (To) for melting (H), cooling… read more here.
Keywords: heating freeze; treated starches; freeze thawing; mild heating ... See more keywords
Efficient production of fungal chitosan utilizing an advanced freeze-thawing method; quality and activity studies
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DOI: 10.1016/j.foodhyd.2018.03.010
Abstract: Abstract Fungal chitosan was produced from brown Agaricus bisporus, white Agaricus bisporus, and Pleurotus ostreatus mushrooms (commercially known as brown portobello, white protobello, and oyster mushrooms, respectively) by utilizing a classic alkali deacetylation protocol (PI),… read more here.
Keywords: freeze thawing; chitosan; protocol; fungal chitosan ... See more keywords
Effects of the combination of freeze-thawing and enzymatic hydrolysis on the microstructure and physicochemical properties of porous corn starch
Sign Up to like & getrecommendations! Published in 2018 at "Food Hydrocolloids"
DOI: 10.1016/j.foodhyd.2018.04.041
Abstract: Abstract The effects of the combination of freeze-thawing and enzymatic hydrolysis on the microstructure and physicochemical properties of porous corn starch were investigated. The combined treatment produced more pores in each starch granule than either… read more here.
Keywords: treatment; freeze thawing; starch; enzymatic hydrolysis ... See more keywords
Relationship between nuclear DNA fragmentation, mitochondrial DNA damage and standard sperm parameters in spermatozoa of fertile and sub‐fertile men before and after freeze‐thawing procedure
Sign Up to like & getrecommendations! Published in 2018 at "Andrologia"
DOI: 10.1111/and.12998
Abstract: The aim of this study was to assess the stability of nuclear and mitochondrial DNA (n‐DNA and mt‐DNA) of spermatozoa under freeze‐thawing and to find out the correlation between them and their association with standard… read more here.
Keywords: freeze thawing; dna; standard sperm; sperm parameters ... See more keywords
Relationship of aquaporins 3 (AQP3), 7 (AQP7), and 11 (AQP11) with boar sperm resilience to withstand freeze–thawing procedures
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DOI: 10.1111/andr.12410
Abstract: Cryopreservation is the most suitable method to preserve boar spermatozoa over long‐term storage. However, freeze–thawing protocols inflict extensive damage to sperm cells, reducing their viability and compromising their fertilizing ability. In addition, high individual variability… read more here.
Keywords: freeze thawing; aqp3 aqp7; boar sperm; aqp7 aqp11 ... See more keywords
Utilization of SUMO tag and freeze-thawing method for high level expression and solubilization of recombinant human angiotensin-converting enzyme 2 (rhACE2) protein in E. coli.
Sign Up to like & getrecommendations! Published in 2022 at "Protein and peptide letters"
DOI: 10.2174/0929866529666220715101357
Abstract: BACKGROUND SARS-CoV-2 uses angiotensin-converting enzyme 2 (ACE2) as a receptor for entering the host cells. Production of the ACE2 molecule is important because of its potency to use as a blocker and therapeutic agent against… read more here.
Keywords: rhace2; level; expression; thawing method ... See more keywords
Freeze-Thawing Treatment as a Simple Way to Tune the Gel Property and Digestibility of Minced Meat from Red Swamp Crayfish (Procambarus clarkiix)
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DOI: 10.3390/foods11060837
Abstract: The effects of freezing methods, including rapid freezing (RF) or slow freezing (SF), combined with thawing methods, e.g., water immersing thawing (WT) or cold thawing (CT), on the meat yield, drip loss, gel properties, and… read more here.
Keywords: red swamp; swamp crayfish; thawing treatment; meat ... See more keywords
Pulsed Electric Field and Freeze-Thawing Pretreatments for Sugar Uptake Modulation during Osmotic Dehydration of Mango
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DOI: 10.3390/foods11172551
Abstract: Osmotic dehydration kinetics depends on food tissue microstructure; thus, modulation of mango porosity could help selectively enhance water removal over sugar gain. In this present study, pretreatments of freeze-thawing (freezing at −36 °C for 2… read more here.
Keywords: electric field; dehydration; osmotic dehydration; pulsed electric ... See more keywords
Antioxidant effects of quercetin in freeze-thawing process of mouse spermatogonial stem cells
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DOI: 10.4103/2305-0500.250417
Abstract: Objective: To evaluate the antioxidant effect of quercetin on cell viability, reactive oxygen species (ROS) contents and apoptosis of cryopreserved mouse spermatogonial stem cells (mSSCs). Methods: mSSCs were isolated from neonate mice and cultivated in… read more here.
Keywords: freeze thawing; spermatogonial stem; thawing process; mouse spermatogonial ... See more keywords