Spectral imaging of FRET‐based sensors reveals sustained cAMP gradients in three spatial dimensions
Sign Up to like & getrecommendations! Published in 2018 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23572
Abstract: Cyclic AMP is a ubiquitous second messenger that orchestrates a variety of cellular functions over different timescales. The mechanisms underlying specificity within this signaling pathway are still not well understood. Several lines of evidence suggest… read more here.
Keywords: camp; fret; spatial dimensions; spectral imaging ... See more keywords
Joint refinement of FRET measurements using spectroscopic and computational tools.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.01.011
Abstract: The variability of the orientation factor is a long-standing challenge in converting FRET efficiency measurements into donor-acceptor distances. We propose the use of molecular dynamics (MD) simulations to characterize orientation distributions and thus improve the… read more here.
Keywords: orientation; fret; computational tools; donor acceptor ... See more keywords
Investigating cardiac β-adrenergic nuclear signaling with FRET-based biosensors.
Sign Up to like & getrecommendations! Published in 2020 at "Annales d'endocrinologie"
DOI: 10.1016/j.ando.2020.04.001
Abstract: By activating membrane β-adrenergic receptors (β-AR), noradrenaline and adrenaline are the most powerful stimulators of cardiac function. β-ARs are coupled to the synthesis of cAMP, which activates the cAMP-dependent protein kinase (PKA). PKA regulates the… read more here.
Keywords: activation; cardiac adrenergic; nuclear signaling; adrenergic nuclear ... See more keywords
Rhodamine B derivative-modified up-conversion nanoparticle probes based on fluorescence resonance energy transfer (FRET) for the solid-based detection of copper ions
Sign Up to like & getrecommendations! Published in 2019 at "RSC Advances"
DOI: 10.1039/c9ra05504e
Abstract: A novel solid-based up-conversion FRET sensor is convenient for the direct detection of Cu2+ based on color change and emission spectra. read more here.
Keywords: detection; solid based; fret; rhodamine derivative ... See more keywords
Resource for FRET-Based Biosensor Optimization
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.885394
Abstract: After the development of Cameleon, the first fluorescence resonance energy transfer (FRET)-based calcium indicator, a variety of FRET-based genetically encoded biosensors (GEBs) have visualized numerous target players to monitor their cell physiological dynamics spatiotemporally. Many… read more here.
Keywords: fret based; resource fret; fret; optimization ... See more keywords