Spectral imaging of FRET‐based sensors reveals sustained cAMP gradients in three spatial dimensions
Sign Up to like & getrecommendations! Published in 2018 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23572
Abstract: Cyclic AMP is a ubiquitous second messenger that orchestrates a variety of cellular functions over different timescales. The mechanisms underlying specificity within this signaling pathway are still not well understood. Several lines of evidence suggest… read more here.
Keywords: camp; fret; spatial dimensions; spectral imaging ... See more keywords
How Native Are Peptides After Activation by Collisions or Photons? A Gas‐Phase FRET Study
Sign Up to like & getrecommendations! Published in 2025 at "Helvetica Chimica Acta"
DOI: 10.1002/hlca.202500043
Abstract: Collisions and lasers are often used to study the structures of ionized biomolecules. While structural changes upon collisions have been studied in more detail, interactions with photons mostly comprise fragmentation. It remains unclear to what… read more here.
Keywords: gas phase; fret; study; activation ... See more keywords
Joint refinement of FRET measurements using spectroscopic and computational tools.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.01.011
Abstract: The variability of the orientation factor is a long-standing challenge in converting FRET efficiency measurements into donor-acceptor distances. We propose the use of molecular dynamics (MD) simulations to characterize orientation distributions and thus improve the… read more here.
Keywords: orientation; fret; computational tools; donor acceptor ... See more keywords
Investigating cardiac β-adrenergic nuclear signaling with FRET-based biosensors.
Sign Up to like & getrecommendations! Published in 2020 at "Annales d'endocrinologie"
DOI: 10.1016/j.ando.2020.04.001
Abstract: By activating membrane β-adrenergic receptors (β-AR), noradrenaline and adrenaline are the most powerful stimulators of cardiac function. β-ARs are coupled to the synthesis of cAMP, which activates the cAMP-dependent protein kinase (PKA). PKA regulates the… read more here.
Keywords: activation; cardiac adrenergic; nuclear signaling; adrenergic nuclear ... See more keywords
Rhodamine B derivative-modified up-conversion nanoparticle probes based on fluorescence resonance energy transfer (FRET) for the solid-based detection of copper ions
Sign Up to like & getrecommendations! Published in 2019 at "RSC Advances"
DOI: 10.1039/c9ra05504e
Abstract: A novel solid-based up-conversion FRET sensor is convenient for the direct detection of Cu2+ based on color change and emission spectra. read more here.
Keywords: detection; solid based; fret; rhodamine derivative ... See more keywords
Resource for FRET-Based Biosensor Optimization
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.885394
Abstract: After the development of Cameleon, the first fluorescence resonance energy transfer (FRET)-based calcium indicator, a variety of FRET-based genetically encoded biosensors (GEBs) have visualized numerous target players to monitor their cell physiological dynamics spatiotemporally. Many… read more here.
Keywords: fret based; resource fret; fret; optimization ... See more keywords