A FRET‐Based Fluorescent Probe to Screen Anticancer Drugs, Inhibiting p73 Binding to MDM2
Sign Up to like & getrecommendations! Published in 2020 at "ChemBioChem"
DOI: 10.1002/cbic.202000660
Abstract: The protein p73 acts as a transcription factor, resulting in tumour suppression. MDM2, an oncogenic protein, can negatively influence p73‐mediated apoptosis by binding to p73 transactivation domains (TAD). Inhibition of the protein‐protein interaction between p73… read more here.
Keywords: protein; p73; fret probe; probe ... See more keywords
Imaging of Genetically Encoded FRET-Based Biosensors to Detect GPCR Activity.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1221-7_11
Abstract: A wealth of assays for screening GPCR activity have been developed. Biosensors that employ Förster Resonance Energy transfer (FRET) are specific and enable dynamic measurements. Moreover, FRET biosensors are ideally suited for the analysis of… read more here.
Keywords: gpcr activity; fret based; genetically encoded; biosensors detect ... See more keywords
Real-Time Measurements of Intracellular cAMP Gradients Using FRET-Based cAMP Nanorulers.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2245-2_1
Abstract: 3',5'-cyclic adenosine monophosphate (cAMP) is one of the most important and ubiquitous second messengers in cells downstream of G protein-coupled receptors (GPCRs). In a single cell, cAMP can exert innumerous specific cell functions in response… read more here.
Keywords: time; camp nanorulers; camp; fret based ... See more keywords
Electrophoresis- and FRET-Based Measures of Serpin Polymerization.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7163-3_24
Abstract: Many serpinopathies, including alpha-1 antitrypsin (A1AT) deficiency, are associated with the formation of unbranched polymer chains of mutant serpins. In vivo, this deficiency is the result of mutations that cause kinetic or thermodynamic destabilization of… read more here.
Keywords: based measures; polymerization; fret based; electrophoresis fret ... See more keywords
An Ultrasensitive FRET-based DNA Sensor via the Accumulated QD System Derivatized in the Nano-beads
Sign Up to like & getrecommendations! Published in 2018 at "BioChip Journal"
DOI: 10.1007/s13206-018-2406-x
Abstract: Förster resonance energy transfer (FRET) is extremely sensitive to the separation distance between the donor and the acceptor which is ideal for probing such biological phenomena. Also, FRET-based probes have been developing for detecting an… read more here.
Keywords: accumulated system; system; dna; dna sensor ... See more keywords
Differences in cytosolic glucose dynamics in astrocytes and adipocytes measured by FRET-based nanosensors.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical chemistry"
DOI: 10.1016/j.bpc.2020.106377
Abstract: The cellular response to fluctuations in blood glucose levels consists of integrative regulation of cell glucose uptake and glucose utilization in the cytosol, resulting in altered levels of glucose in the cytosol. Cytosolic glucose is… read more here.
Keywords: astrocytes adipocytes; glucose dynamics; fret based; dynamics astrocytes ... See more keywords
PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.03.003
Abstract: We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement… read more here.
Keywords: pie flim; fret based; living cells; different fret ... See more keywords
FRET-based aptamer biosensor for selective and sensitive detection of aflatoxin B1 in peanut and rice.
Sign Up to like & getrecommendations! Published in 2017 at "Food chemistry"
DOI: 10.1016/j.foodchem.2016.10.004
Abstract: Aflatoxins are potential food pollutants produced by fungi. Among them, Aflatoxin B1 (AFB1) is the most toxic. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, utilizing a FRET-based method,… read more here.
Keywords: detection; rice; aptamer biosensor; fret based ... See more keywords
FRET- based immunoassay using CdTe and AuNPs for the detection of OmpW antigen of Vibrio cholerae
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Luminescence"
DOI: 10.1016/j.jlumin.2017.08.032
Abstract: Abstract In the past few decades, nanoscales Forster resonance energy transfer (FRET) based techniques have been used to improve the bioanalysis applications. In this study a new FRET immunosensor based on CdTe was developed for… read more here.
Keywords: detection; vibrio cholerae; ompw; fret based ... See more keywords
Studying β1 and β2 adrenergic receptor signals in cardiac cells using FRET-based sensors.
Sign Up to like & getrecommendations! Published in 2019 at "Progress in biophysics and molecular biology"
DOI: 10.1016/j.pbiomolbio.2019.06.001
Abstract: Cyclic 3'-5' adenosine monophosphate (cAMP) is a key modulator of cardiac function. Thanks to the sophisticated organization of its pathway in distinct functional units called microdomains, cAMP is involved in the regulation of both inotropy… read more here.
Keywords: camp; based sensors; studying adrenergic; fret based ... See more keywords
Rational design of a FRET-based ratiometric fluorescent chemosensor for detecting ClO− with large Stokes based on rhodamine and naphthalimide fluorophores
Sign Up to like & getrecommendations! Published in 2020 at "Tetrahedron"
DOI: 10.1016/j.tet.2020.131291
Abstract: Abstract The design and synthesis of high selective and sensitive fluorescence chemosensor for the determination of ClO− has received great efforts due to its critically important role in the immune system, however, most of chemosensor… read more here.
Keywords: based ratiometric; chemosensor; detecting clo; fret based ... See more keywords